Abstract: Objective：To optimize the best extraction process of polysaccharides in bamboo leaves.Methods：With polysaccharides of bamboo leaves being an examinable target component，the optimized extraction techniques and condition were chosen by orthogonal test method.Results：The best extraction process of polysaccharides of bamboo leaves was that the crude drugs were extracted with petroleum ether for refluxed 2 h，the sediments extracted with 95% ethanol for 2 h first，then filtrable residue from previous steps were extracted three times with 30 times amount of water.The proteins were removed by Sevage’s method，adding ethanol and making its concentration to 80%，the extracts were stored in the refrigerator for 12 h.The acquired amount of bamboo leaves polysaccharides was about 0.51% from the best extraction process.Conclusion:The extractive rate of bamboo leaves polysaccharides was higher in the optimized extraction process and the process was proved to be reasonable and feasible.

Key words: bamboo leaves, polysaccharides, extraction process