journal6 ›› 2005, Vol. 26 ›› Issue (1): 37-39.

• Doctoral Forum • Previous Articles     Next Articles

Improvement of Determination on TTC-Dehydrogenase Activity in Biofilms


  1. (1.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,Hubei China;2.Graduate School of the Chinese Academy of Sciences,Beijing 100039;3.Biology Department,Hubei Normal University,Huangshi 435002,Hubei China)
  • Online:2005-01-15 Published:2012-09-22

Abstract: The method of 3,5,5-triphenyltetrazolium (TTC) determining biofilms activity was improved,and the problem that the standard curve of determination on TTC-dehydrogenase activity can’t be made conveniently and stably was resolved.The factors which influence the determination were studied.The results showed that sodium sulfide as reducer,toluene as TF organic extractant,Tris-HCl as buffer solution,pH 8.6 and 38 ℃ were the best condition on the developed method,and the optimum reaction time to cultivate biofilms is 6 h.

Key words: biofilms, TTC, dehydrogenase activity, standard curve

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