Abstract: The conditions which prepare high-purity Lecithin with 1. 5 cm×40 cm silica gel chromatographic column are systematically studied. The component of eluent is CH₃OH-CHCl₃ solvents with pH 3~ 4. The optimum gradient elution process is concave gradient eluting with the gradient of V( CH₃OH)∶V( CHCl₃) = ( 1∶2) ~ ( 2∶1) . The volume of eluent is only 5~ 6 times of column volume, and the time of eluting is about 6 h.

Key words: chromatographic process;Lecithin, separating