journal6 ›› 2014, Vol. 35 ›› Issue (5): 63-66.DOI: 10.3969/j.issn.1007-2985.2014.05.015

• Medical Science • Previous Articles     Next Articles

Special Staining Pathologic Changes and the Expression of NF-κB After Diffuse Axonal Injury in Rats

 CHEN  Ren-Hui, FAN  Zhao-Hui, GAO  Hui   

  1. (1.Department of Criminal Sciences and Technology,Fujian Police Institute,Fuzhou 350007,China;2.Criminal Investigation Detachment of Public Security Bureau Cangshan Branch,Fuzhou 350007,China;3.Department of Pharmacology,Medical College of Jishou University,Jishou 416000,Hunan China)
  • Online:2014-09-25 Published:2014-10-30

Abstract: To observe the pathologic changes and the expression of nuclear factor-kappa B(NF-κB)after diffuse axonal injury(DAI) in rats,its mechanism is firstly explored.The rat DAI model was established.Immunohistochemical SABC method was used to observe the expression of NF-κB,combining Glees-Marsland and Weil staining to observe the pathologic changes in different stage of DAI.Swollen and ruptured axon were found in brain tissue 1 h after DAI,and myelin sheath showed edema,delamination,marked axonal retraction ball and screen shape changes were detected after 24 h,and those morphological changes reached the peak after 72 h.The expression of NF-κB could be detected 1h after injury,the positive cells increased after 3 h,peaked after 24 h,and kept this tendency for 72 h.The positive cells decreased after 7 d.The control group showed negative staining.The findings showed that the expression of NF-κB increased in rat DAI model.The NF-κB may play an important role in secondary axonal injury.

Key words: diffuse axonal injury, special staining, nuclear factor-kappa B(NF-&kappa, B), immunohistochemistry

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