Technical Conditions for Ultrasound-Assisted Extraction of Protein of Eucommia Seed

doi:10.3969/j.issn.1007-2985.2014.01.018

Abstract

Abstract: The optimum conditions for the ultrasound-assisted extraction of Eucommia seed protein were studied by single factor and orthogonal test method.The solid-liquid ratio,ultrasonic time,ultrasonic temperature,extraction pH value were selected as single factor to study experimental conditions of Eucommia seed protein extraction process,and then optimum process conditions were determined by L9(34) orthogonal test .The experimental results showed that the optimum conditions for ultrasound-assisted extraction of Eucommia seed proteins were as follows:solid to liquid ratio was 1∶25,ultrasonic time 30 min,ultrasonic temperature at 50 ℃,extraction pH value of 10.0.Under this condition,extraction rate of Eucommia seed protein was 77.03%.

Key words: protein of eucommia seed, ultrasound-assisted extraction, orthogonal test

HUANG Cheng, YIN Hong. Technical Conditions for Ultrasound-Assisted Extraction of Protein of Eucommia Seed[J]. journal6, 2014, 35(1): 78-82.

References

[1]江苏中医学院.中药大辞典[M].上海：上海科技出版社,1977：1 031-1 033.

[2]王俊丽.杜仲研究[M].河北:河北大学出版社,1992：46-73.

[3]罗士苇.杜仲的经济价值[J].植物生理学通讯,1956(2):25-26.

[4]汤诗杰,李和平,贺善安.杜仲研究的现状与展望[J].林业科技开发,2007,21(2):8-12.

[5]汪俊超.杜仲栽培技术[J].现代农业科技,2011(27):147-148.

[6]刘世会,赵德刚,宋宝安.杜仲皮蛋白对植物病原真菌的抑制作用[J].农药,2007,46(12):848- 850.

[7]汪盛,向烨,李根培,等.杜仲抗真菌蛋白( EAFP) 晶体动态生长研究[J].华中师范大学学报:自然科学版,2007,41(2):249-253.

[8]刘世会,赵德刚.杜仲皮蛋白对霉菌的抑制作用[J].食品科学,2008,29(3):149-151.

[9]张羽,郑铁松,陈静.莲子蛋白质提取工艺研究[J].食品科学,2007,28(9):144-147.

[10]张文英,党斌,孙小凤.超声波辅助提取菜籽粕中蛋白的工艺研究[J].粮油加工,2011,6:69-71.

[11]黄群,麻成金,孙术国,等.超声波辅助提取蚕豆蛋白及其功能特性研究[J].食品与发酵工业,2009,35 (8):179-182.

[12]金晶,徐志宏,魏振承,等.超声微波辅助法提纯菜籽蛋白的研究[J].现代食品科技,2009,25(3):275-278.

[13]赵玉红,李莉.超声波辅助提取松仁蛋白的工艺研究[J].中国林副特产,2008,92:6-8.

[14]王钦德,杨坚.食品实验设计与统计分析[M].中国农业大学出版社,2011.

[15]马丹.凯氏定氮法测定食品中蛋白质含量[J].计量与测试技术,2008（35）:57-58.

[16]JEBRAIL M J,WHEELER A R.Digital Microfluidic Method for Protein Extraction by Precipitation[J].Analytical Chemistry,2009,81(1):330~335.

[17]BERNARDI RA,NEGRI A,RONCHI S,et al.Isolation of the Epithiospecifier Protein from Oil-Rape (Brassica Napus Ssp.Oleifera) Seed and Its Characterization[J].FEBS Letters,2000,467(23):296-298.