Abstract: Paris Saponin II,VI,H were used as reference substances,and the rhizomes of Paris fargesii,Trillium tschonoskii,and Paris (The rhizome of Paris polyphylla Smith var. chinensis (Franch.) Hara and Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz.) were identified by thin-layer chromatography (TLC).The results showed that the three reference substances had high resolution and clear spots on the silica gel G thin-layer plate with chloroform-methanol-water (15∶5∶1) as the developing agent under the conditions of 20 ℃±2 ℃ and 50%~60%.According to the difference in components,TLC can be used to distinguish Paridis fargesii rhizome,Trillium tschonoskii rhizome,and Paris rhizome.

Key words: Paridis fargesii rhizome, Trillium tschonoskii rhizome, Paris rhizome, Paris Saponin Ⅱ, Paris Saponin Ⅵ, Paris Saponin H, thin layer chromatography