有毒药用植物野八角叶绿体基因组特征及系统发育分析

doi:10.13438/j.cnki.jdzk.2025.03.008

摘要/Abstract

摘要：为了揭示有毒药用植物野八角的叶绿体基因组特征，分析野八角与八角属其他物种间的亲缘关系，利用二代测序技术对野八角叶片进行高通量测序，经组装、注释后得到完整的叶绿体基因组序列.采用生物信息学方法对野八角叶绿体基因组序列特征进行分析，并基于叶绿体基因组及CDS序列构建野八角与八角属内物种的系统发育树.结果表明，野八角叶绿体基因组的序列全长为143 012 bp，共编码112个基因，包括78个蛋白编码基因、29个tRNA基因、4个rRNA基因和1个假基因，总GC摩尔分数为39.1%；野八角叶绿体基因组共有100个简单重复序列，以单核苷酸重复居多；八角属植物叶绿体基因组变异程度均呈现出LSC区和SSC区高于IRs区，5个高变区分别是trnK-UUU-rps16，accD-psal，rpl20-clpP1，ycf1，atpF；八角属中，野八角与中缅八角亲缘关系最近，支持率高达100%，且野八角与八角同属一个单系类群，亲缘关系较近.

关键词： 野八角, 八角属, 叶绿体基因组, 系统发育, 亲缘关系

Abstract: In order to explore the chloroplast genome characteristics of the toxic medicinal plant Illicium simonsii and analyze its relationships with other species of the Illicium,this study used next-generation sequencing (NGS) technology to perform high-throughput sequencing for leaf material of Illicium simonsii,and obtained the complete chloroplast genome after assembly and annotation,which was analyzed using bioinformatics.Meanwhile,phylogenetic tree of Illicium simonsii and the related species in the Illicium genus were constructed based on chloroplast genomes and coding sequences (CDS).The result showed that the full length of the Illicium simonsii chloroplast genome was 143 012 bp,which encoded 112 genes,including 78 protein-coding genes,29 tRNA genes,4 rRNA genes,and 1 pseudogene,with a total GC molar fraction of 39.1%;there were a total of 100 simple sequence repeats (SSRs) in the chloroplast genome of Illicium simonsii,with the largest number of mono-nucleotides.The degree of chloroplast genomic variation in Illicium plants all showed higher LSC and SSC regions than IRs regions,and the five highly variable regions were trnK-UUU-rps16，accD-psal，rpl20-clpP1，ycf1，atpF;Within the genus Illicium,Illicium simonsii was most closely related to Illicium burmanicum,with a support rate as high as 100%,and both Illicium simonsii and Illicium verum belong to the same monophyletic clade,exhibiting closer phylogenetic affinity.

Key words: Illicium simonsii, Illicium, complete chloroplast genome, phylogeny, genetic relationship

高恬恬, 毛玖阳, 李海峰, 张德全. 有毒药用植物野八角叶绿体基因组特征及系统发育分析[J]. 吉首大学学报（自然科学版）, 2025, 46(3): 62-74.

GAO Tiantian, MAO Jiuyang, LI Haifeng, ZHANG Dequan, 3. Characterization and Phylogenetic Analysis of the Chloroplast Genome of the Toxic Medicinal Plant Illicium simonsii[J]. Journal of Jishou University(Natural Sciences Edition), 2025, 46(3): 62-74.

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