毛茛铁线莲叶绿体全基因组解析及系统发育分析

doi:10.13438/j.cnki.jdzk.2024.01.012

摘要/Abstract

摘要：为了解析药用植物毛茛铁线莲的叶绿体全基因组特征及系统发育位置，利用高通量测序技术对毛茛铁线莲叶片样品进行测序，并对毛茛铁线莲叶绿体基因组进行组装、注释和特征分析，采用最大简约法（MP）、最大似然法（ML）及贝叶斯法（BI）构建分子系统发育树.结果表明，毛茛铁线莲的叶绿体基因组为典型的四分体结构，全长159 741 bp，共编码112个基因，包括79个蛋白编码基因、29个tRNA基因和4个rRNA基因；毛茛铁线莲叶绿体基因组共含有84个简单重复序列，以单核苷酸重复基序居多，共57个；毛茛铁线莲叶绿体基因组使用频次最高的氨基酸是亮氨酸；基于叶绿体全基因组构建的MP，ML和BI树，拓扑结构基本一致；铁线莲属是明显的单系类群，与银莲花属关系最近，毛茛铁线莲与绣球藤亲缘关系最近.

关键词： 毛茛铁线莲, 叶绿体基因组, 简单重复序列, 系统发育, 毛茛科

Abstract: To reveal the chloroplast genome characteristics of Clematis ranunculoides and its phylogenetic relationships,the present study adopted the Illumina platform to acquire high-throughput sequences,which were used for assemble,annotate,and characterize the complete chloroplast genome of C. ranunculoides.And then molecular phylogenetic trees were constructed with maximum parsimony (MP),maximum likelihood (ML) and Bayesian (BI) methods.The result shows that the total length of the chloroplast genome of C. ranunculoides is 159 741 bp,which encodes 112 genes,including 79 protein-coding genes,29 tRNA genes and 4 rRNA genes;there are 84 SSRs in the chloroplast genome of C. ranunculoides,and 57 of them are mono-nucleotide.Analysis of codon usage bias reveales that leucine is the most frequently utilized amino acid in the chloroplast genome.The topological structures are basically identical among MP,ML,and BI trees.Derived from the phylogenetic trees,Clematis is a monophyletic group,closely related to Anemone.And C. ranunculoides is closest to C. montana,which supportes the traditional classification results.

Key words: Clematis ranunculoides, chloroplast genome, simple sequence repeats, phylogeny, Ranunculaceae

王若澜, 张德全. 毛茛铁线莲叶绿体全基因组解析及系统发育分析[J]. 吉首大学学报（自然科学版）, 2024, 45(1): 66-76.

WANG Ruolan, ZHANG Dequan. Complete Chloroplast Genome of Clematis ranunculoides:Characterization and Phylogeny Analysis[J]. Journal of Jishou University(Natural Sciences Edition), 2024, 45(1): 66-76.

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