纳木错牦牛粪便中纤维素降解菌的鉴定及产酶特征

doi:10.13438/j.cnki.jdzk.2023.03.009

吉首大学学报（自然科学版） ›› 2023, Vol. 44 ›› Issue (3): 61-65.DOI: 10.13438/j.cnki.jdzk.2023.03.009

纳木错牦牛粪便中纤维素降解菌的鉴定及产酶特征

李强，罗金，阳佳宁，高宏涛，刘璇，贺建武

（1.吉首大学生物资源与环境科学学院，湖南吉首 416000；2.吉首大学杜仲综合利用技术国家地方联合工程实验室，湖南吉首 416000）

出版日期:2023-05-25 发布日期:2023-06-16
通讯作者: 贺建武（1985—），男，湖南溆浦人，吉首大学生物资源与环境科学学院高级实验师，博士，主要从事生物资源及传统知识可持续利用研究.E-mail:hejsu@jsu.edu.cn.
基金资助:
湖南省科技厅高新技术产业科技创新引领计划项目（2020SK2028）；湖南省教育厅科学研究项目（18C0576）

Identification of Cellulose Degrading Bacteria in Namco Yak Dung and Its Enzyme Production Characteristics

LI Qiang,LUO Jin,YANG Jianing,GAO Hongtao,LIU Xuan,HE Jianwu

(1.College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China;2.National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides,Jishou University,Jishou 416000,Hunan China)

Online:2023-05-25 Published:2023-06-16

摘要/Abstract

摘要：通过富集、纯化从西藏纳木错的牦牛粪便中筛选得到2株高效纤维素降解菌株，采用滤纸条崩解试验测定不同培养温度、培养时间和初始pH值条件下纤维素降解菌株的产酶特征，并初步分析了目标菌株的遗传地位.结果表明，菌株X1和X2具有显著降解纤维素优势，两菌株在培养温度9 ℃、培养时间6 d、初始pH值4的条件下，产出的纤维素降解酶具有较强的相对酶活性.系统发育分析显示，X1和X2可能为Cronobacter属菌株.

关键词： 纤维素降解菌, 纤维素酶, 牦牛粪便, 系统发育分析

Abstract: Through enrichment and purification,high efficient cellulose degrading strains were screened from yak dung in Namco Lake,Tibet.The enzyme production characteristics of cellulose degrading strains under different conditions were determined,and the genetic differences of the target strains were analyzed.The results showed that strain X1 and X2 had significant cellulose degradation advantage,and the relative enzyme activities of the two strains were strong at pH 4 and 9 ℃.Phylogenetic analysis showed that X1 and X2 might belong to the genus Cronobacter.

Key words: cellulose degrading bacteria, cellulase, yak dung, phylogenetic analysis

李强, 罗金, 阳佳宁, 高宏涛, 刘璇, 贺建武. 纳木错牦牛粪便中纤维素降解菌的鉴定及产酶特征[J]. 吉首大学学报（自然科学版）, 2023, 44(3): 61-65.

LI Qiang, LUO Jin, YANG Jianing, GAO Hongtao, LIU Xuan, HE Jianwu. Identification of Cellulose Degrading Bacteria in Namco Yak Dung and Its Enzyme Production Characteristics[J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(3): 61-65.

[1]	刘彪, 马娟, 谭玲, 林永慧, 王海燕, 刘任成. 利用曲霉sp.HX-1发酵稻草秸秆制备纤维素酶[J]. journal6, 2012, 33(4): 95-100.
[2]	张迎庆, 干信, 谢笔钧. 纤维素酶制备魔芋葡甘低聚糖[J]. journal6, 2003, 24(3): 42-44.

纳木错牦牛粪便中纤维素降解菌的鉴定及产酶特征

Identification of Cellulose Degrading Bacteria in Namco Yak Dung and Its Enzyme Production Characteristics

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