云南澜沧江水系高体鳑鲏的遗传多样性分析

doi:10.13438/j.cnki.jdzk.2024.04.008

摘要/Abstract

摘要：为了探究云南澜沧江水系高体鳑鲏的遗传多样性，采用聚合酶链式反应（PCR）产物直接测序法测定了采自云南省西双版纳段澜沧江水系的10条高体鳑鲏鱼类样本的线粒体细胞色素C氧化酶亚基Ⅰ（COI）基因片段.此外，结合GenBank数据库中6种鳑鲏属鱼类COI基因的同源序列，进行了遗传多样性、遗传距离和系统发育分析.结果表明，云南澜沧江水系高体鳑鲏的COI基因序列片段长度为657 bp，碱基A+T含量（52.5%）大于G+C含量（47.5%），序列变异位点21个，单倍型4个；云南澜沧江水系高体鳑鲏的单倍型多样性指数值0.711，核苷酸多样性指数值0.008 2，平均核苷酸差异数值5.400，中性检验Tajima's D值-1.353 78（P>0.10）；云南澜沧江水系高体鳑鲏与保山高体鳑鲏间的遗传距离最小（0.005），与济南鳑鲏间的遗传距离最大（0.196）；云南澜沧江水系高体鳑鲏与保山高体鳑鲏的序列聚为单系支（支持率100%），苦味鳑鲏与科尔沁达鳑鲏、南方鳑鲏聚为一支，济南鳑鲏、朝鲜鳑鲏均单独聚为一个单系支.

关键词： 高体鳑鲏, COI基因, 遗传多样性, 系统发育关系

Abstract: To investigate genetic diversity of Rhodeus ocellatus from Lancang River of Yunnan,this study applied PCR amplification and sequencing methods to determine the mitochondrial COI gene sequence fragments of 10 R. ocellatus samples from Xishuangbanna.Additionally,we combined with the homologous sequence of COI gene of six Rhodeus species from GenBank database to execute the genetic diversity,genetic distance and phylogenetic analyses.Sequence analyses showed that the COI gene sequence fragments was 657 bp,the base composition A+T content (52.5%) was larger than the G+C content (47.5%),and there were 21 variable sites and 4 haplotypes.The haplotype diversity of R. ocellatus was 0.711,the nucleotide diversity was 0.008 2,the average nucleotide difference was 5.400,and the Tajima's D value was -1.353 78 (P>0.10).The minimum genetic distance between our R. ocellatus samples and R. ocellatus from Baoshan city was 0.005,and the maximum genetic distance between R. ocellatus samples and R. notatus was 0.196.Phylogenetic analysis showed that R. ocellatus samples were clustered into a monophyletic branch with the same sequence published from Baoshan city with high bootstrap value (100%).Furthermore,the R. amarus,R. colchicus and R. meridionalis samples clustered together as a monophyletic clade,while R. notatus and R. uyekii samples were gathered to form a monophyletic branch.

Key words: Rhodeus ocellatus, COI gene, genetic diversity, phylogenetic relationship

李蓉, 宁艳红, 孟飞燕, 徐伟江, 田瀚吉, 范丽仙. 云南澜沧江水系高体鳑鲏的遗传多样性分析[J]. 吉首大学学报（自然科学版）, 2024, 45(4): 50-58.

LI Rong, NING Yanhong, MENG Feiyan, XU Weijiang, TIAN Hanji, FAN Lixian. Genetic Diversity of Rhodeus Ocellatus from Lancang River of Yunnan[J]. Journal of Jishou University(Natural Sciences Edition), 2024, 45(4): 50-58.

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