乌金猪mtDNA D-Loop区的遗传多样性

doi:10.3969/j.cnki.jdxb.2018.02.014

吉首大学学报（自然科学版）

乌金猪mtDNA D-Loop区的遗传多样性

王志敏，徐亚欧，孙艳，杨磊，徐珑洋，王英明，黄卫平

（1.西南民族大学生命科学与技术学院，四川成都 610041；2.凉山州畜牧兽医科学研究所，四川凉山州 615042）

出版日期:2018-03-25 发布日期:2018-04-28
通讯作者: 黄卫平,女(藏族),凉山州畜牧兽医科学研究所高级畜牧师,主要从事养猪生产与科研工作.
作者简介:王志敏(1989—),女,贵州人,西南民族大学生命科学与技术学院硕士生,主要从事动物遗传育种研究
基金资助:
凉山州应用技术研究与开发项目（13CXY0004）

Genetic Diversity of mtDNA D-Loop Sequences in Wujin Pig

WANG Zhimin,XU Yaou,SUN Yan,YANG Lei,XU Longyang, WANG Yingming,HUANG Weiping

(1.College of Life Science and Technology,Southwest MinzuUniversity,Chengdu 610041,China;2.Animal Husbandry and Veterinary Research Institute of Liangshan Yi Autonomous Prefecture,Liangshan zhou 615042,SichuanChina)

Online:2018-03-25 Published:2018-04-28

摘要/Abstract

摘要：

为揭示乌金猪3个地方类群遗传多样性及其分布情况.以乌金猪的3个地方类群凉山猪、大河猪和柯乐猪为对象，研究了线粒体DNA Ｄ环高变区（mtDNA D-loop HVI）的序列多态性.研究表明，在全长290 bp的核苷酸序列中共检测到46个突变位点，其中简约信息位点为5个，单一多态位点为37个，其中以A/G突变为主.凉山猪的3个类群（昭觉、美姑、西昌）首先聚为一类，然后凉山猪与大河猪聚为一类，最后凉山猪与柯乐猪聚类.3个地区的乌金猪有2个母系起源，大河猪与凉山猪为一个母系起源，柯乐猪为另一个母系起源.就总体而言，乌金猪的各地方类群凉山猪与大河猪遗传亲缘关系最近，其次是柯乐猪，有2个母系起源.

关键词： 乌金猪, mtDNA D-loop, 遗传多样性

Abstract:

To reveal the genetic and distribution of the three native pigs of Wujin pig,this paper investigates the mitochondrial DNA D-loop region (mtDNA D) of three native pigs of Wujin pig.The Results showed that 46 polymorphism sites were found in the full length 290 bp.Among them，there are 5 simple informative loci and 37 single polymorphic loci，among which A/G mutation is the main one.Three groups of Liangshan pig (Zhaojue,Meigu,Xichang) clustered together firstly,and then Liangshan pig clustered together with Dahe pig，and finally Liangshan pig clustered together with Kele pig.Three native pigs of Wujin pigs had 2 maternal origins,Dahe pig and Liangshan pig originated in a matriarchal,Kele pig originated from another maternal line.The genetic relationship between Liangshan pig and the Dahe pig is closer.There are two maternal origins.

Key words: Wujin pig, mtDNA D-loop, genetic diversity

王志敏，徐亚欧，孙艳，杨磊，徐珑洋，王英明，黄卫平. 乌金猪mtDNA D-Loop区的遗传多样性[J]. 吉首大学学报（自然科学版）, DOI: 10.3969/j.cnki.jdxb.2018.02.014.

WANG Zhimin,XU Yaou,SUN Yan,YANG Lei,XU Longyang, WANG Yingming,HUANG Weiping. Genetic Diversity of mtDNA D-Loop Sequences in Wujin Pig[J]. Journal of Jishou University（Natural Sciences Edition), DOI: 10.3969/j.cnki.jdxb.2018.02.014.

乌金猪mtDNA D-Loop区的遗传多样性

Genetic Diversity of mtDNA D-Loop Sequences in Wujin Pig

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