吉首大学学报(自然科学版)

• 生物与化学化工 • 上一篇    下一篇

乳酸杆菌产胞外多糖培养条件的优化

宋昊芮,李彦,王乐,姚一帆   

  1. (长沙理工大学化学与食品工程学院,湖南 长沙 410114)
  • 出版日期:2019-09-25 发布日期:2019-11-12
  • 通讯作者: 李彦(1970—),女,长沙理工大学化学与食品工程学院副教授,主要从事食品微生物、农副产品加工与营养、益生菌研究.
  • 基金资助:

    湖南省教育厅一般项目(18C0233);湖南省教育厅大学生研究性学习和创新性实验计划项目(S201910536030)

Optimum Cultivation Conditions for Extracellular Polysaccharide in Lactobacillus

SONG Haorui, LI Yan, WANG Le, YAO Yifan   

  1. (College of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, China)
  • Online:2019-09-25 Published:2019-11-12

摘要:

从自制泡菜汁中提取乳杆菌,经生化鉴定确认其细菌菌属,再从乳酸杆菌中提取EPS并用苯酚-硫酸法进行多糖含量的测定。在温度、pH值、葡萄糖含量和实验时间单因素实验的基础上,采用四因素三水平正交试验对LAB EPS产量的影响进行了研究,最终确定泡菜中乳酸杆菌获得高产胞外多糖的最佳条件和最佳组合为,温度35 ℃,葡萄糖质量分数1.5%,pH值6.5,发酵时间10 h.

关键词: 乳酸杆菌, 分离鉴定, 优化, 胞外多糖

Abstract:

The lactobacillus extracted from homemade Sauerkraut juice was firstly biochemically identified for its bacterial species,, and then EPS extracted from the lactobacillus was determined by phenol-sulfuric acid method for its polysaccharide content. Based on single factor test, effects of temperature, pH value, glucose content, and experimental time on LAB EPS yield were studied by the orthogonal test with four factors at three different levels. The optimum conditions for high yield of extracellular polysaccharide in lactobacillus are as follows: 35 ℃, 1.5% mass fraction for glucose, pH 6.5, and 10 h fermentation.

Key words: lactobacillus, isolation and identification, optimization, extracellular polysaccharide

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