Content of Medicinal Chemistry and Pharmacology in our journal

Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Purification Process of Total Flavonoids from Lilium lancifolium Thunb and Its Antibacterial Activity ZHU Lanping, YUAN Jiao, YUAN Jieying, YIN Xinlian, LIU Ying, CHENG Jiang, YAO Shufeng Journal of Jishou University(Natural Sciences Edition)    2024, 45 (6): 24-30.   DOI: 10.13438/j.cnki.jdzk.2024.06.004 Abstract （478）      PDF（pc） （915KB）（359）       Knowledge map    Save Through comparing the static adsorption-desorption performance of 7 kinds of macroporous resins,the best macroporous resin with high adsorption rate and desorption rate was selected.The optimal purification process of Lilium lancifolium by macroporous resin was selected by single factor experiment and orthogonal experiment,and the total flavonoid content and antibacterial activity before and after purification were determined.The results showed that the macroporous resin with high adsorption and desorption performance was AB-8.The optimal purification process was as follows:the total flavonoid concentration of the feed solution was 0.076 4 g/L,the pH value was 4,the adsorption time was 8 hours,the solvent volume fraction was 60%,the solvent volume was 120 mL,and the desorption time was 10 hours.The total flavonoid content after purification was 11.55%,which was 5.3 times that of the purification before.The inhibition zone diameter of the purified total flavonoid was larger than that of the total flavonoid before purification,and the inhibitory effect on Staphylococcus aureus was higher than that on Escherichia coli. Reference | Related Articles | Metrics | Comments（0） Select Extraction Process and Antibacterial Activity of Total Saponins from Rhizomes of Rohdea chinensis GUO Wei, MAO Jiayi, XU Liang, QING Dujiang, CHENG Jiang, YAO Shufeng Journal of Jishou University(Natural Sciences Edition)    2024, 45 (6): 31-36.   DOI: 10.13438/j.cnki.jdzk.2024.06.005 Abstract （380）      PDF（pc） （790KB）（135）       Knowledge map    Save The total saponin was isolated from Rohdea chinensis rhizome through the ethanol water bath heating extraction method.The influences of ethanol concentration,liquid to material ratio,extraction time and extraction temperature on the yield rate of the total saponin were investigated.On the basis of single factor test,the orthogonal test was used to optimize the extraction process.The optimal extraction conditions of total saponins from the rhizome of Rohdea chinensis are as follows:material-liquid ratio 1∶30 (g∶mL),ethanol concentration 60%,extraction time 60 min,and extraction temperature 80 ℃.Under these extraction conditions,the total saponin yield of Rohdea chinensis was 17.46%.The results of the antibacterial activity test showed that the total saponins in the rhizome of the Rohdea chinensis had inhibitory effects on Escherichia coli,Staphylococcus aureus,Candida albicans,and the inhibitory activity on Staphylococcus aureus was the best,with the diameter of the inhibition circle 12 mm at a concentration of 5 g/L. Reference | Related Articles | Metrics | Comments（0） Select Optimization of Extraction Process and Vitro Antioxidant Activity of Total Polysaccharides from Polygonatum odoratum ZHANG Yan, ZHANG Yang, CHEN Jiarui, YAO Shufeng, CHENG Jiang Journal of Jishou University(Natural Sciences Edition)    2024, 45 (6): 37-43.   DOI: 10.13438/j.cnki.jdzk.2024.06.006 Abstract （569）      PDF（pc） （6072KB）（238）       Knowledge map    Save The extraction process and antioxidant activity of total polysaccharide from the rhizome of Polygonatum odoratum were evaluated.The total polysaccharide was isolated from Polygonatum odoratum rhizome through ultrasound-assisted water bath heating extraction method.The influences of material-liquid ratio,ultrasonic power,ultrasonic extraction temperature on the extraction rate of the total polysaccharide were investigated.Based on the results of single-factor experiments,the response surface experiments give the optimal extraction conditions of total polysaccharide from the rhizome of Polygonatum odoratum∶ material-liquid ratio 1∶15 (g∶mL),ultrasonic power 300 W,extraction time 60 min,and ultrasonic extraction temperature 60 ℃.Under these extraction conditions,the total polysaccharide yield of Polygonatum odoratum was 13.58%.The results of the antioxidant activity test showed that when the mass concentration of Polygonatum odoratum polysaccharide was 2.75 g/L,the scavenging rates of Polygonatum odoratum polysaccharide on DPPH radicals and OH radicals were 34.18% and 62.04%,respectively. Reference | Related Articles | Metrics | Comments（0） Select Mechanism of Caulis Sinomenii in Treating Rheumatoid Arthritis Based on Network Pharmacology and Molecular Docking WU Ziqi, GONG Bufan, WANG Jianchao, PENG Huayong Journal of Jishou University(Natural Sciences Edition)    2024, 45 (6): 44-53.   DOI: 10.13438/j.cnki.jdzk.2024.06.007 Abstract （624）      PDF（pc） （19913KB）（280）       Knowledge map    Save To study the mechanism of Caulis Sinomenii in the treatment of rheumatoid arthritis,network pharmacology and molecular docking were used to screen 6 main active ingredients of Caulis Sinomenii from the TCMSP database.268 drug targets corresponding to the active ingredients were obtained through the PubChem library and Swiss Target Prediction.Subsequently,5 706 targets for rheumatoid arthritis were obtained through GeneCards,DisGeNET,and OMIM databases.By utilizing the Venny 2.1.0 platform to draw Venn diagrams,178 intersection targets between the active ingredients of Caulis Sinomenii and rheumatoid arthritis diseases were identified.A network diagram illustrating the interaction between the active ingredients of Caulis Sinomenii and rheumatoid arthritis disease was constructed using Cytoscape 3.10.0.Additionally,a drug component target protein network diagram was created.GO enrichment analysis and KEGG pathway enrichment analysis were conducted using the DAVID database.Molecular docking simulation was performed between the target and the active ingredients of the drug using Autodock software.The results revealed that NR3C1,AKT1,SRC,PPARG,and JUN are the key target proteins for the anti-rheumatoid arthritis effect of Caulis Sinomenii.The GO enrichment analysis results indicated that biological processes mainly include G protein-coupled receptor signaling pathway,coupled to cyclic nucleotide second messenger G,vascular process in circulatory system,adenylate cyclase-modulating G protein-coupled receptor,peptidyl-serine phosphorylation,etc.The cellular components mainly include integral component of presynaptic membrane,intrinsic component of presynaptic membrane,membrane raft,membrane microdomain,etc.The molecular functions mainly involve nuclear receptor activity,ligand-activated transcription factor activity,catecholamine binding,protein tyrosine kinase activity,etc.According to the KEGG enriched bubble diagram,it can be inferred that the affected pathways include the cancer pathway,the neuroactive ligand receptor interaction pathway,and the inflammatory mediator regulatory pathway of the TRP channel.The molecular docking results indicated that the active ingredients,including β-sitosterol,razmanine,xylosterol,sinomenine,and levodontidine,are associated with the core targets NR3C1,AKT1,SRC,PPARG,and JUN,all showing good binding activity.The treatment of rheumatoid arthritis with Caulis Sinomenii may involve a multi-component,multi-target,and multi-pathway process,which includes regulating inflammatory cytokines,immune cells,cell cycles (such as apoptosis and burning),and osteoblasts and osteoclasts to treat rheumatoid arthritis. Reference | Related Articles | Metrics | Comments（0）