Screening and Identification of a High-Efficiency Cyclohexanone-Degrading Strain JDM-3-11

Abstract

Abstract: This paper reported screening a strain numbered JDM-3-11 for effectively degrading cyclohexanone,which was acclimated and isolated from the sludge from Baling Petrochemical Company in Yueyang.The strain was tentatively identified as Rhodococcus ruber by bacterial morphological observation,physiological and biochemical test,and 16S rRNA sequences analysis.The study showed that when the content of cyclohexanone was 2000 mg/L,the temperature was 30 ℃,the rotate speed was 150 r/min and pH was 7,the cyclohexanone biodegrading rate of strain JDM-3-11 within 70 hours reached 94.79%.

Key words: cyclohexanone, biodegradation, 16S rRNA gene sequence, Rhodococcus ruber

HE Qian, LIU Zhu-Xiang, YI Lang-Bo, XIAO Huai-Dong, HUANG Ke, CHEN Yi-Guang, PENG Qing-Jing. Screening and Identification of a High-Efficiency Cyclohexanone-Degrading Strain JDM-3-11[J]. journal6, 2008, 29(2): 92-95.

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