journal6 ›› 2009, Vol. 30 ›› Issue (5): 77-81.

• Biological resources • Previous Articles     Next Articles

Cloning and Correlation Analysis of Cold Adaptation of NADH-Quinone Oxidoreductase 3 Subunit Gene from Common Carp(Cyprinus Carpio)


  1. (1.College of Electrical & Electronic Engineering,Harbin University of Science and Technology,Harbin 150080,China;2.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;3.College of Life Sciences,Nankai University,Tianjin 300071,China)
  • Online:2009-09-25 Published:2012-04-21

Abstract: Genes expressed specifically at low temperature were used to design primers and to screen brain full-length cDNA library of common carp (Cyprinus carpio) through two rounds of clonal PCR method.After that,gene 152 was discovered and sequenced,and determined finally as NADH-quinone oxidoreductase 3 subunit gene using homologous searches.This gene was confirmed to play an important role as the force generators for fish in a low water environment.In addition,NADH-quinone oxidoreductase 3 subunit gene was associated with cold adaptation of common carp to some extent in oxidation of respiratory chain electron transport system.

Key words: common carp(Cyprinus carpio), NADH-quinone oxidoreductase 3 subunit gene, cloning, cold adaptation

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