journal6 ›› 2016, Vol. 37 ›› Issue (1): 74-79.DOI: 10.3969/j.cnki.jdxb.2016.01.017

• Biological and chemical • Previous Articles     Next Articles

Content Determination and Ultrasonic Extraction Techniques of Total Flavonoids from Capsellae Bursa-Pastoris (L.)

 LIU  Ya-Qian, WEI  Hua, LI  Gui, HE  Jian-Wu, CHEN  Tao, ZHANG  Yuan, ZHOU  Fen   

  1. (1.College of Biological Resources and Environmental Sciences,Jishou University,Jishou 416000,Hunan China;2. Key Laboratory of Plant Resourses Conservation and Utilization,Jishou University,Jishou 416000,Hunan China;3. Laboratory and Equipment Administration Center,Jishou University,Jishou 416000,Hunan China)
  • Online:2016-01-25 Published:2016-01-21

Abstract: UV spectrophotometry for determination of total flavonoids was used.Via ultrasonic extraction technique,using NaNO3-Al(NO3) to detect the absorbance of samples and taking lutin as comparison,the contents of total flavonoids were determined.On the basis of single factor experiments,the orthogonal experiment was used in the optimization of technological parameters:extracting temperature,ethanol concentration,extracting time,the ratio of solvent to raw materials.The result showed that the contents of total flavonoids in different kinds of Capsella bursa-pastoris L. were from 0.49% to 3.83%.The contents of total flavonoids in flowers & fruits were the highest,and then in leaves and stems.The contents of total flavonoids in cultivated varireties were higher than those in wile varireties.The optimum extraction conditions were as follows:temperature at 65 ℃,50% ethanol concentration,ultrasonic extraction for 40 min,and the  25∶1 ratio of solvent to raw materials.In these conditions,the total flavonoids extraction rate was 89.26%.

Key words: Capsellae bursa-pastoris (L.), total flavonoids, orthogonal experiments, ultrasonic extraction technology

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