Classification and Identification of the Erysipelothrix Species Strains Based on the groEL Gene Sequences

doi:10.3969/j.cnki.jdxb.2015.04.014

Abstract

Abstract: To investigate the effect of 16S rRNA gene and groEL gene used in the classification and identification of species of the genus Erysipelothrix，the 16S rRNA and groEL genes of four E.rhusiopathiae strains were amplified by PCR and sequenced and their phylogenetic threes was constructed.The open reading frame of groEL genes of four tested strains were 1 614 bp in length，and sequence similarities of the groEL gene between the four tested strains and the previously reported E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 were 99% and 86%，respectively.While the 16S rRNA genes of the four tested strains was 1 351 bp in length，and sequence similarity of the 16S rRNA gene between the four tested strains and E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 was 99%.Phylogenetic analysis indicated that the E.rhusiopathiae and E.tonsillarum were differentiated well by groEL gene sequence analysis.This study demonstrated that the groEL gene is an alternative phylogenetic marker for the classification of species of the genus Erysipelothrix.

Key words: Erysipelothrix rhusiopathiae；Erysipelothrix tonsillarum；16S rRNA, groEL；classification and determination

YANG Zhenlong，NAZIERBIEKE Wulumuhan，BORRATHYBAY Enotmack. Classification and Identification of the Erysipelothrix Species Strains Based on the groEL Gene Sequences[J]. journal6, 2015, 36(4): 54-58.

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