journal6 ›› 2013, Vol. 34 ›› Issue (5): 85-88.DOI: 10.3969/j.issn.1007-2985.2013.05.021

• Biological resources • Previous Articles     Next Articles

Cloning and Expression of spaA Gene of Erysipelothrix Rhusiopathiae C43065

LIU  Dan-Dan, YANG  Zhen-Long, WU  Lu-Mu-Han-·Na-Zi-尔Bie-Ke   

  1.  (College of Biology and Environmental Sciences,Jishou University,Jishou 416000,China)
  • Online:2013-09-25 Published:2013-11-04

Abstract: The spaA gene encoding mature surface protective antigen A (SpaA) without signal peptide was amplified from genomic DNA of E.rhusiopathiae C43065 by PCR,The BamHⅠand HindⅢ digested PCR product was cloned into prokaryotic expression vector pET32a to generate a recombinant plasmid pET-spaA.The recombinant protein rSpaA was expressed in E.coli BL21 harboring the recombinant plasmid pET-spaA by IPTG inducing,and the expressed protein was determined by SDS-PAGE.The DNA sequence analysis showed that the spaA gene of C43065 strain was 1794 bp in length.SDS-PAGE analysis revealed a single protein band with a molecular weight of 86 kDa successfully expressed in E.coli BL21.The expressed protein of rSpaA will contribute to further study on protective domain of this protein.

Key words: Erysipelothrix rhusiopathiae, spaA gene, cloning, prokaryotic expression

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