Abstract: A RP-HPLC method for the determination of cefradin in raw drug was established.A column of YQG-ODS was used with a mobile phase of V(CH₃OH）∶V(4%HAc-NaAc）＝3∶7 solution.The UV detector was set at 262 nm.The external standard method was used.There was a good linear correlation in the range of 0.027～0.29  g·L^-1,Y（μV·ｓ）=1.88×107X(g·L^-1)+20　876,r=0.998　3.The average recovery was 99.7%,RSD was 0.57%（n=3）.The elapsed time of a chromatographic analysis was 12 min.The method is rapid,accurate and reliable.

Key words: RP-HPLC, cefradin, external standard method