journal6 ›› 2005, Vol. 26 ›› Issue (4): 29-33.

• Biological resources • Previous Articles     Next Articles

Effect  of Purification of  Echinococcus Antigen on Immune Response with Specific Immunoglobulin G


  1. (College of Life Sciences,Beijing Normal University 100875,Beijing China)
  • Online:2005-10-15 Published:2012-09-17

Abstract: The level of specific immunoglobulin G (IgG) in sera of hydatidosis patients against Echinococcus granulosus antigen is detected in dot-immunobinding assay with purified and crude Echinococcus granulosus antigens.The experiment carried out with purified antigen and the serum 1∶200 obtains higher specificity (98.9%);meanwhile,the experiment maintains the higher sensitivity (93.7%) which is obtained in the experiment carried out with crude antigen and the serum dilution 1∶400.These results indicate that in the research of specific immunoglobulin G in sera of hydatidosis patients anti-Echinococcus granulosus antigen,higher sensitivity (93.7%) and higher specificity (98.4,98.9%) can be obtained with purified Echinococcus granulosus antigen and crude Echinococcus granulosus antigen respectively;higher specificity is achieved when purified antigen is used,but there is no significant difference between purified and crude antigen in statistics.

Key words: hydatidosis;echinococcus granulosus antigen;immunoglobulin G;immune , response;dot immunobinding assay

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