Detection of the Level of mIL- 2R and T Cells Subsets in PBMC in Patients With Hepatitis C

Abstract

Abstract: To study the level of membrane interleukin- 2 receptor ( mIL- 2R) and T cell subsets in PBMC in patients with hepatitis C and its role in the pathogenesis of hepatitis C, the level of mIL- 2R and T cells subsets in peripheral blood mononuclear cells ( PBMC) were detected with biotin- streptatividin technique before and after PHA induction in 203 cases of hepatitis C with positive HCV- RNA, ant i- HCV( + ) , ant i- HCV( - ) . The total results of the expressive level of mI L- 2R before and after PHA inducting and T cell subsets in PBMC were all lower in hepatitis C than those in normal controls ( P < 0. 01) .Among them, the levels of mI L- 2R were lower in silence than those in situation of PHA induction ( P < 0. 01) . The levels of CD⁺₃ , CD⁺₄ , CD⁺₄ / CD⁺₈ in acute and chronic hepatitis C with ant i- HCV( + ) were lower and CD⁺₈ was higher than those in normal controls ( P < 0. 01) .The function of cellular immunity, the levels of mI L- 2R and activation of T cells are obviously in disorder in patients with hepatitis C, and those were closely as sociated with changing of the hepatitis into chronic.

Key words: hepatitis C, membrane interleukin- 2 receptor, T- cell subsets, PBMC

WANG Ke-Xia, LI Chao-Pin, WANG Jian. Detection of the Level of mIL- 2R and T Cells Subsets in PBMC in Patients With Hepatitis C[J]. journal6, 2001, 22(4): 79-82.

References

[ 1] 王健,张荣波, 许礼发,等. HCV 侵染患者外周血单个核细胞后对细胞免疫功能的影响[ J] . 安徽医科大学学报, 1999,34 ( 2) : 103.

[ 2] 吴海滨,窦晓光, 曲以琴.慢性丙型肝炎患者外周血单核细胞中丙型肝炎病毒 RNA 正、负链的检测[ J] . 中国公共卫生,1997, 13 ( 5) : 270.

[ 3] 王健,李朝品. HBV 感染乙肝患者 PBMC后对细胞免疫功能的影响[ J] .中国人兽共患病杂志, 1998, 14 ( 6) : 90- 92.

[ 4] 朱睛晖,陆俊茜, 吴海辉,等. 用 BSA 系统免疫细胞化学法检测人外周血 T和 B淋巴细胞[ J] . 上海医学检验杂志, 1993,8 ( 4) : 201- 211.

[ 5] SAROBE P. Producti on of Interleukin- 2 in Response to Syn thetic Peptides From Hepatitis C Viral in PatientsWith Chronic HepatitisC: Relationship Wi th Response to Interferon Treatment[ J] . J. Hepatol, 1996, 25( 1) : 1.

[ 6] KOZIEL M J, DUDLEY D, AFDHAL N, et al. Hepatitis C Virus ( HCV) - speci fic Cytotoxic T Lymphocytes Recognize Epitopes in the Core and Envelope Proteins of HCV[ J] . J. Virol. , 1993, 67: 7522.

[ 7] 王健,李朝品, 许礼发.丙肝患者外周血 T 细胞亚群及mIL- 2R 的检测[ J] . 蚌埠医学院学报, 1999, 24 ( 5) : 305- 307.

[ 8] MULLER H M, PFAFF E, GOEFFER T, et al. Peripheral Leukocytes Serve as a Possible Extrahelatic Site for Hepatitis Cvirus Replication[ J] . J. Gen. Virol. , 1993, 74 ( 4) : 669- 676.

[ 9] WILLEMS M, PEERLINCK K, MOSHAGE H, et al.Hepatitis C Virus- RNA i n Plasma and i n Peripheral BloodMononuclear Cells of Hemophiliacs With Chronic Hepatitis C: Evidence for Virus Replication in Peripheral BloodMononuclear Cells[ J] . J. Med. , V irol. , 1994, 42 ( 3) : 272- 278.

[ 10] 叶红军,杨世忠, 李石,等. 可溶性白介素- 2受体在慢性乙型肝炎中的意义及其升高机理初探[ J] . 中华消化杂志,1994, 14 ( 1) : 86.

[ 11] HOFFMANN RM, DIEPOLDER HM, ZACHOVAL R, et al. Mapping of Immunodominant CD+4 T Lymphocyte Epitopes of Hepatitis C Virus Anti gens and Their Relevance During the Course of Chronic Infection[ J] . Hepatology, 1995, 21: 632.

[ 12] LIAW Y F, LEE C S, TSAI C L, et al. T- cell- mediated Autologous Hepatocytotoxicity in Patients Wi th Chronic Hepati tis C Virus Infection[ J] .Hepatology, 1995, 22: 1.

[ 13] PHAM B N, MOSNIER J F, WALKER F, et al. Flow Cytometry CD+4 / CD+8 Ratio of Liver- derived Lymphocytes Correlates With Viral Replication in Chronic Hepati tis B[ J] . Clin. Exp. Immunol. , 1994, 97: 403.
[ 14] BOTARELLI P, BRUNETTO M R, MINUTELLO M A, et al. T- lymphocyte Response to Hepati tis C Virus in Di fferent Clinical Courses of Infection[ J] . Hepatolgy, 1993, 104: 580.

[ 15] KNODELL R G, ISHACK K G, BLACK W C, et al. Formulati on and Application of a Numerical Scori ng System for Assessing Histo - logical Activity in Asmptomatic Chronic Active Hepatiti s[ J] . Hepatology, 1981, ( 1) : 431.