journal6 ›› 2001, Vol. 22 ›› Issue (3): 16-21.

• Scientific research bulletin • Previous Articles     Next Articles

RAPD Marking and Its Application in Forest Genetics and Tree Breeding

  

  1. (Ministerial Key Lab of Economic Forest Tree Breeding and Cultivation, South Central Forestry University, Zhuzhou 412006, Hunan China)
  • Online:2001-09-15 Published:2013-01-05

Abstract: RAPD marking is derived from PCR methodology and procedures. It is similar to RFLP, SSR and AFLP to some extent but is obviously different from them in many aspects. One disadvantage of RAPD marking exists in the relatively lo wreproducibility, but its reliability as genetic markers has been verified in alarge number of e xperiments.Optimizing the lab conditions and experimental protocols has proved to be instrumental in promoting the results reproducibility. RAPD marking has been extensively used in forest genetic studies and tree breeding trials, including construction of genetic linkage maps, analysis of population genetic st ructure and diversity, study of evolution systems,taxonomic identification, DNA fingerprinting and improvement of genetic traits.

Key words: RAPD marking, forest genetics and tree breeding, literature review

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