Abstract: Because the fruiting rate and germinat ive percentage of Gerbe rajame sonii Bolus are lower, tissue culture was explored to solve the propagating problem in the product ion. The results show that the shoot-tip is the best mate rial in speeding propagation among the three explants ( shoot- tip, stem and petiole) as it has the bigges tinductivity,the best growth and it is easy to be inducted adventitious buds.The suitable phytohormone compositions toninduct callus, adventitious buds and roots are respectively: MS+ BA 1. 0 mg•L^-1+ IBA 0. 05mg•L^-1+sugar 3.0%, Ms+BA2. 0 mg•L^-1+ IAA 0. 5 mg•L^-1+ sugar 3. 0% or Ms+ Kt 3. 0 mg•L^-1+ IAA 0. 05 mg•L^-1+ sugar 3. 0% , and Ms+ NAA 0. 3 mg•L^-1+ IBA 0. 3 mg•L^-1+ sugar 3. 0%.

Key words: Gerbera jamesonii Bolus, tissue culture, callus, adventitious bud