Abstract: Spores of Dolichomitriopsis diversiformis were planted on Knop medium with two kinds of cultures (submerged culture and normal culture).The germinations of the spores were very similar in these two conditions and the germination rates were over 90%.The protonema in the submerged condition,which was of slow growth,great length and a few branches,couldn’t produce gametophore primordium even at day 80.While,the protonema in the normal condition,which was of fast growth and many branches,produced gametophore primordium at day 40.The results showed that the submerged culture has an obvious effect on the development of protonema.

Key words: submerged culture, protonema, gametophore primordium