Surface-Modification of CdTe Quantum Dots with Bovine Serum Albumin and Analytical Application

Abstract

Abstract: Mercaptoacetic acid-stabilized CdTe quantum dots were prepared by hydrothermal synthesis method.The CdTe quantum dots exhibited absorption at 502 nm and fluorescence emission at 538 nm,respectively.When surface-modification of CdTe quantum dots were replaced with BSA,absorption peak and the fluorescence peak remained the same,while absorption intensity and the fluorescence emission intensity increased.The effect of pH、temperature and ion strength on fluorescence intensity were discussed.Under the optimal conditions,a mass concentration of BSA in the range of 0.05~1.0 mg/L shows a linear relationship with the fluorescence intensity increasing value of CdTe quantum dots,with a detection limit of 0.011 7 mg/L.The proposed method was applied to detection of the concentration of BSA in buffer solution.

Key words: CdTe, quantum dots, bovine serum albumin, surface-modification

ZHANG Wen-Long, ZHANG Jun-Sheng, ZHOU Li-Ping, CHEN Li-Hua. Surface-Modification of CdTe Quantum Dots with Bovine Serum Albumin and Analytical Application[J]. journal6, 2011, 32(1): 93-97.

References

[1] WARREN C W C,NIE S M.Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection [J].Science,1998,281(5 385):2 016-2 018.

[2] MITCHELL G P,MIRKIN C A,LETSINGER R L.Programmed Assembly of DNA Functionalized Quantum Dots [J].J. Am. Chem. Soc.,1999,121(35):8 122-8 123.

[3] MATTOUSSI H,MAURO J M,GOLDMAN E R,et al.Self-Assembly of CdSe-ZnS Quantum Dot Bioconjugates Using an Engineered Recombinant Protein [J].J. Am. Chem. Soc.,2000,122(49):12 142-12 150.

[4] SUN B Q,XIE W Z,YI G S,et al.Microminiaturized Immunoassays Using Quantum Dots as Fluorescent Label by Laser Confocal Scanning Fluorescence Detection [J].J. Immuno Methods,2001,249(1/2):85-89.

[5] BRUCHEZ M,MORONNE M,GIN P,et al.Semiconductor Nanocrystals as Fluorescent Biological Labels [J].Science,1998,281(5 385):2 013-2 016.

[6] 张春阳,马辉,丁尧,等.量子点标记天花粉蛋白的研究 [J].高等学校化学学报,2001,22(1):34-37.

[7] 林章碧,张皓,陈奇丹,等.利用水相合成的量子点标记木瓜蛋白酶的研究 [J].高等学校化学学报,2003,24(4):609-611.

[8] 陈莉华,覃事栋,李朝阳.胃蛋白酶对CdTe纳米粒子的表面修饰及分析应用 [J].高等学校化学学报,2007,29(2):277-282.

[9] 马金杰,梁建功,韩鹤友.不同巯基试剂修饰的CdTe量子点与BSA相互作用研究 [J].光谱学与光谱分析,2010,30(4):1 039-1 043.

[10] 杨冬芝,孙世安,陈启凡,等.CdSe量子点与蛋白质的作用研究 [J].激光生物学报,2007,16(5):527-531.

[11] 梁佳然,钟文英,于俊生.高质量CdTe量子点的水相快速合成 [J].高等学校化学学报,2009,30(1):14-18.

[12] XIE H Y,LIANG J G,ZHANG Z L,et al.Luminescent CdSe-ZnS quantum Dots as Selective Cu2+ Probe [J].Spectrochim Acta Part A:Molecular & Biomolecular Spectroscopy,2004,60(11):2 527-2 530.

[13] ZHONG W Y,LIANG J R,YU J S.Systematic Study of the Interaction of Cobalt Ions with Different-Sized CdTe Quantum Dots [J].Spectrochimica Acta Part A:Molecular & Biomolecular Spectroscopy,2009,74(3):603-606.