journal6 ›› 2006, Vol. 27 ›› Issue (3): 117-118.

• 化学化工 • 上一篇    下一篇

心脉通滴丸中葛根素含量的HPLC法测定

  

  1. (1.湖南湘泉制药有限公司,湖南 吉首 416000;2.北京燕化凤凰医院药剂科,北京 102500)
  • 出版日期:2006-05-25 发布日期:2012-09-11
  • 作者简介:彭咏梅(1969-),女,湖南省保靖县人,湖南湘泉制药有限公司医药工程师;何咏梅(1970-),女,北京市人,北京燕化凤凰医院药剂科副主任药剂师.

Determination of Puerarin in Xingmaitong Drop Pills by HPLC

  1. (1.Hunan Xiangquan Pharmaceutical Ltd.,Jishou 416000,Hunan China;2.Medicament Office of Bejing Yanhua Fenghuang Hospital,Bejing 102500,China)
  • Online:2006-05-25 Published:2012-09-11

摘要:使用高效液相色谱法测定心脉通滴丸中葛根素的含量,结果表明:在4.16~83.2 mg/L范围内,进样浓度与峰面积值有良好的线性关系,y=3 814.6x-12.716,R2=0.999 9;平均回收率为100.84%,RSD为1.06%.该法准确,重现性好,效率高,操作简便.

关键词: 心脉通滴丸, 葛根素, 高效液相色谱法

Abstract: Objective To establish a method for determination of Puerarin in xingmaitong.Method HPLC was used,the separation was performed on Luna  C18 (5 μg,250 mm×4.6 mm)  column,a  mixture of  methanol-water(25∶75) was servered  as the mobile phase,and the  detecting wavelength was at 2 250 nm.Results The linearity range was from 4.16~83.2 mg/L,and the mean recovery was 100.84%,RSD 1.06%.Conclusion The method is simple,accurate and good for the determination of Puerarin in Xingmaitong,and it can be used for quality control for Xingmaitong.

Key words: Xingmaitong drop pills, puerarin, HPLC

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