journal6 ›› 2015, Vol. 36 ›› Issue (4): 48-53.DOI: 10.3969/j.cnki.jdxb.2015.04.013

• 生物与化学 • 上一篇    下一篇

湘杂油631油菜种子黄、黑粒的耐贮性差异

李亮,米泽洲,张祥,李晓明,田向荣   

  1. (1.吉首大学植物资源保护与利用湖南省高校重点实验室,湖南 吉首 416000;2.湘西土家族苗族自治州农业科学研究院,湖南 吉首 416000;3.吉首大学生物资源与环境科学学院,湖南 吉首 416000)
  • 出版日期:2015-07-25 发布日期:2015-07-27
  • 作者简介:李亮(1970—),男,湖南常德人,湘西土家族苗族自治州农业科学研究院农艺师,主要从事种子评价与推广研究.
  • 基金资助:

    国家自然科学基金资助项目(31360062);吉首大学生物食品类专业湖南省大学生创新训练中心资助项目(JDCX2013-12)

Storability Differences Between Yellow and Black Seeds of Xiangzayou 631

 LI  Liang, MI  Ze-Zhou, ZHANG  Xiang, LI  Xiao-Ming, TIAN  Xiang-Rong   

  1. (1.Key University Laboratory of Plant Resources Conservation and Utilization (Hunan Province),Jishou University,Jishou 416000,Hunan China;2.Institute of Agricultural Science,Xiangxi Autonomous Prefecture of Tujia and Miao Minorities,Jishou 416000,Hunan China;3.College of Biology and Environmental Science,Jishou University,Jishou  416000,Hunan China)
  • Online:2015-07-25 Published:2015-07-27

摘要:为探讨油菜种子颜色与耐贮性的关系,以高温(40 ℃)高湿(100%)人工老化湘杂油631黄、黑粒种子为材料,分析不同老化时间下的萌发特性和抗氧化生理生化变化.研究结果表明,在人工老化加深的条件下,湘杂油631黄、黑粒种子的萌发率均显著下降,且黄籽的生活力下降明显快于黑籽;人工老化3 d的种子相对于未老化的,O-2产生速率和H2O2含量均有大幅度提升,脂质过氧化终产物MDA含量明显提升;抗氧化SOD和CAT活性均有显著提升,AsA含量明显下降;老化过程中,黄粒种子的O-2产生速率和H2O2含量均高于黑粒种子,SOD活性和AsA含量明显低于黑粒种子,湘杂油631黑粒种子比黄粒种子具有更好的耐贮性,其耐贮性主要依赖于黑粒种子含有活性更多的非酶抗氧化物质.

关键词: 油菜, 种子, 黄黑粒, 耐贮性, 差异

Abstract: To investigate the relationship between seed color and storability,the artificial aged Xiangzayou 631 seeds have been used as materials to study the germination and antioxidative physiological and biochemical variation.Results showed that the germination rate of both yellow and black rape seeds decreased significantly after accelerated artificial aging,and germination of yellow seeds decreased dramatically faster than the black. Superoxide radicle production,contents of hydrogen peroxide and MDA increased by 3 d accelerated artificial aging treatment,activities of antioxidative enzyme (SOD and CAT) increased obviously,and the content of AsA declined obviously.The comparison between two color seeds showed superoxide radicle production,hydrogen peroxide contents of the yellow seeds were much higher than those of the black seeds,but the SOD activity and AsA content of yellow seeds were much lower than those of the black seeds.It can be concluded that the black seeds of Xiangzayou 631 possess stronger storability due to greater contents of  nonenzymatic antioxidants.

Key words: Xiangzayou 631, yellow and black, seeds, storability, difference

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