基于groEL基因序列鉴定丹毒丝菌属菌种

doi:10.3969/j.cnki.jdxb.2015.04.014

journal6 ›› 2015, Vol. 36 ›› Issue (4): 54-58.DOI: 10.3969/j.cnki.jdxb.2015.04.014

基于groEL基因序列鉴定丹毒丝菌属菌种

杨振龙, 吾鲁木汗·那孜尔别克, 恩特马克·布拉提白

（1.吉首大学生物资源与环境科学学院，湖南吉首 416000；2.伊犁师范学院生物与地理科学学院，新疆伊宁 835000）

出版日期:2015-07-25 发布日期:2015-07-27
通讯作者: 吾鲁木汗·那孜尔别克（1961—），女，新疆塔城托里人，新疆伊犁师范学院生物与地理科学学院教授，博士，主要从事病原微生物致病机理研究.
作者简介:杨振龙（1988—），男，湖南长沙人，吉首大学生物资源与环境科学学院硕士生，主要从事病原微生物学研究
基金资助:
国家自然科学基金资助项目（31072142，31360613）；湖南省科技厅科技计划资助项目（2009FJ3215）

Classification and Identification of the Erysipelothrix Species Strains Based on the groEL Gene Sequences

YANG Zhenlong，NAZIERBIEKE Wulumuhan，BORRATHYBAY Enotmack

(1.College of Biology and Environmental Sciences，Jishou University，Jishou 416000，Hunan China;2.College of Biological and Geography Sciences，Yili Normal University，Yining 835000，Xinjiang China)

Online:2015-07-25 Published:2015-07-27

摘要/Abstract

摘要：对4株红斑丹毒丝菌的16S rRNA基因和groEL基因进行测序及系统发育分析，探讨groEL基因序列分析法在丹毒丝菌属菌种分类鉴定中的应用.测序结果表明，4株红斑丹毒丝菌groEL基因长度为1 614 bp，编码由537个氨基酸残基组成热休克蛋白HPS60，与红斑丹毒丝菌ATCC19414株和扁桃体丹毒丝菌ATCC43339株groEL基因的同源性分别为99%和86%，而16S rRNA基因长度为1 351 bp，与红斑丹毒丝菌ATCC19414株和扁桃体丹毒丝菌ATCC43339株的同源性均为99%.系统发育分析结果表明，groEL基因比经典的16S rRNA基因序列分析分辨率高，可适用于红斑丹毒丝菌和扁桃体丹毒丝菌的分类鉴定.

关键词： 红斑丹毒丝菌, 扁桃体丹毒丝菌, 16S rRNA基因, groEL基因, 分类鉴定

Abstract: To investigate the effect of 16S rRNA gene and groEL gene used in the classification and identification of species of the genus Erysipelothrix，the 16S rRNA and groEL genes of four E.rhusiopathiae strains were amplified by PCR and sequenced and their phylogenetic threes was constructed.The open reading frame of groEL genes of four tested strains were 1 614 bp in length，and sequence similarities of the groEL gene between the four tested strains and the previously reported E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 were 99% and 86%，respectively.While the 16S rRNA genes of the four tested strains was 1 351 bp in length，and sequence similarity of the 16S rRNA gene between the four tested strains and E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 was 99%.Phylogenetic analysis indicated that the E.rhusiopathiae and E.tonsillarum were differentiated well by groEL gene sequence analysis.This study demonstrated that the groEL gene is an alternative phylogenetic marker for the classification of species of the genus Erysipelothrix.

Key words: Erysipelothrix rhusiopathiae；Erysipelothrix tonsillarum；16S rRNA, groEL；classification and determination

杨振龙, 吾鲁木汗·那孜尔别克, 恩特马克·布拉提白. 基于groEL基因序列鉴定丹毒丝菌属菌种[J]. journal6, 2015, 36(4): 54-58.

YANG Zhenlong，NAZIERBIEKE Wulumuhan，BORRATHYBAY Enotmack. Classification and Identification of the Erysipelothrix Species Strains Based on the groEL Gene Sequences[J]. journal6, 2015, 36(4): 54-58.

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基于groEL基因序列鉴定丹毒丝菌属菌种

Classification and Identification of the Erysipelothrix Species Strains Based on the groEL Gene Sequences

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