Journal of Jishou University(Natural Sciences Edition)

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Determination and Analysis of Complete Mitochondrial Genome of Ozobranchus jantseanus: Based on Pacbio and Illumina Sequencing

GU Shengli, LIU Jianjun, SUN Entao, ZHAN Xiaodong, NIE Liuwang   

  1. (1. College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui China;  2. Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, Anhui China)
  • Online:2020-03-25 Published:2020-12-13


The complete mitochondrial genome of Ozobranchus jantseanus was sequenced and annotated accurately through a combination of Illumina and Pacbio technology, and the comparative analysis of the gene order and phylogenetic relationship with other species in the Hirudinea were performed. The results showed that the complete mitochondrial genome length of O. jantseanus was 14 868 bp, and there were two kinds of start codon, ATG and ATT, and four types of stop codon, TAA, TAG, TA and T in the coding gene of O. jantseanus. Comparative analysis showed that mitochondrial genes orders of 10 species in the Hirudinea were divided into two types, and the differences were reflected in the exchange of tRNA gene order and the length of long noncoding region (LNR). All Rhynchobdellida species, including O. jantseanus, belonged to the b-type, and most of the Arhynchobdellida species to a-type. The Neighbor-Joining (NJ) evolution analysis of 13 protein coding genes (PCGs) from species in the Hirudinea showed that O. jantseanus and others species in Rhynchobdellid formed a monophyletic group.

Key words: Ozobranchus jantseanus, complete mitochondrial genome, Hirudinea, Pacbio and Illumina sequencing

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