Abstract: This paper aims at comparing the contents of total flavonoids in the leaves of Phyllostachys heterocycla cv. Pubescens from different regions in Xingxi.Ethanol extraction was used to extract total flavonoids from bamboo leaves,rutin was used as controller and the total flavonoid contents in the leaves of Phyllostachys heterocycla cv. Pubescens from five different regions in Xiangxi(Guzhang，Longshan，Jishou，Huayuan，Yongding) were determined by ultraviolet spectrophotometer.The total flavonoids content in the bamboo leaves from Huayuan was found to be the highest at 3.49%,and that from Longshan was the lowest at 1.32%.The contents of flavonoids in the bamboo leaves from Guzhang,Jishou,Yongding were at 3.04%，2.54%，1.86% respectively.It can be concluded that the contents of total flavonoids in bamboo leaves were different in different regions.Among them,the medical value of bamboo leaves in Huayuan is the highest.

Key words: Xiangxi area, bamboo leaves, total flavonoids