胃蛋白酶对CdS纳米粒子的表面修饰及分析应用

journal6 ›› 2008, Vol. 29 ›› Issue (2): 96-100.

胃蛋白酶对CdS纳米粒子的表面修饰及分析应用

(1.吉首大学化学化工学院,湖南吉首 416000；2.湖南大学化学生物传感与计量学国家重点实验室，湖南长沙 410082；3.吉首大学湖南省林产化工工程重点实验室,湖南张家界 427000)

出版日期:2008-03-25 发布日期:2012-05-25
作者简介:陈莉华(1963-), 女，博士，吉首大学化学化工学院教授，主要从事生物化学分析及纳米粒子在化学上的应用.
基金资助:
湖南省教育厅自科基金重点项目(05A009)；湖南大学化学生物传感与计量学国家重点实验室开放课题(2005019)；吉首大学博士基金资助项目

Surface-Modification of CdS Nano-Particles by Using Pepsin and Its Analytical Application

(1.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China;2.State Key Laboratory of Chemo/Biosensing and Chemometrics,Hunan University,Changsha 410082,China;3.Hunan Province Key Laboratory of Forest Product Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,Hunan China)

Online:2008-03-25 Published:2012-05-25

摘要/Abstract

摘要：以巯基乙酸为稳定剂及表面修饰剂,在水溶液中合成了平均粒径为10 nm左右的CdS纳米粒子,用胃蛋白酶改变CdS纳米粒子的表面修饰状态并研究了其系列特性.CdS纳米粒子在292 nm附近有强的紫外吸收，有524.8 nm的荧光发射，胃蛋白酶对其表面修饰后，紫外吸收峰位不变，荧光峰位蓝移至462.4 nm,荧光强度降低.温度及pH值对表面修饰产生影响.在最佳实验条件下,胃蛋白酶质量浓度在2～20 mg/L范围内与荧光降低值之间成线性关系,检测限 (3σ) 为0.13　mg/L (n=10)，方法可用于人体胃液胃蛋白酶的测定.

关键词： CdS纳米粒子, 胃蛋白酶, 表面修饰, 荧光测定

Abstract: CdS semiconductor nano-particles were synthesized in aqueous solution by using mercaptoethylic acid as the stabilizer as well as surface-modification reagent.The average size of CdS nano-particles estimated from TEM graph is ca.10 nm.The CdS nano-particles exhibited strong ultraviolet absorption at 292 nm and fluorescence emission at 524.8 nm.When surface-modification of CdS nano-particles were replaced with pepsin,ultraviolet absorption peak kept same,fluorescence emission intensity decreased as well as fluorescence peak shifted to 462.4nm.Temperature and pH value influenced surface-modification of CdS nano-particles.Under optimal conditions,a concentration of 2～20 mg/L of pepsin could be determined on the basis of fluorescence intensity decrease ratio of CdS nano-particles,with a detection limit 3σ of 0.13 mg/L (n=10).The proposed method was applied to the detection of the concentration of pepsin in human gastric juice.

Key words: CdS nano-particles, pepsin, surface-modification, fluorescence determination

陈莉华, 覃事栋, 卜晓英, 陈益超. 胃蛋白酶对CdS纳米粒子的表面修饰及分析应用[J]. journal6, 2008, 29(2): 96-100.

CHEN Li-Hua, QIN Shi-Dong, BU Xiao-Ying, CHEN Yi-Chao. Surface-Modification of CdS Nano-Particles by Using Pepsin and Its Analytical Application[J]. journal6, 2008, 29(2): 96-100.