journal6 ›› 2016, Vol. 37 ›› Issue (1): 64-68.DOI: 10.3969/j.cnki.jdxb.2016.01.015

• 生物与化学 • 上一篇    下一篇

蛇足石杉芽胞萌发及生长发育的影响因素

骆冰洁,姚茂君,曹德华   

  1. (1.吉首大学林产化工工程湖南省重点实验室,湖南 张家界 427000;2.南京大学生命科学学院,南京 210093)
  • 出版日期:2016-01-25 发布日期:2016-01-21
  • 通讯作者: 姚茂君(1968—),男,湖南吉首人,吉首大学林产化工工程湖南省重点实验室教授,主要从事天然产物和功能食品开发研究.
  • 作者简介:骆冰洁(1990—),女,湖南常德人,吉首大学林产化工工程湖南省重点实验室硕士生,主要从事森林食品加工与应用研究
  • 基金资助:

    国家自然科学基金资助项目(31360161);国家发改委国家生物医药高技术产业化资助项目(发改委高技[2007]2490 号文件)

Factors of Influencing Gemmae Germination and Growth of Huperzia Serrata

 LUO  Bing-Jie, YAO  Mao-Jun, CAO  De-Hua   

  1. (1.Hunan Province Key Laboratory of Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,Hunan China;2.School of Life Science,Nanjing University,Nanjing 210093,China)
  • Online:2016-01-25 Published:2016-01-21

摘要:为研讨蛇足石杉芽胞萌发及生长发育的影响因素,试验分析了培养温度、培养基和光照强度以及赤霉素对蛇足石杉芽胞萌发及生长发育的影响.试验结果表明,蛇足石杉芽胞萌发最佳培养温度为(22±2)℃;最佳培养基为Moore,萌发率约82%,植株平均高度约14.620 mm;Knudson C培养基次之,萌发率约64%,植株平均高度约为5.158 mm;暗培养适宜芽胞萌发及不定根生长,萌发率高达92%,平均根长9.088mm,800 lx弱光照培养不定根生长缓慢,平均根长8.675 mm,1 600 lx光照对不定根的生长有一定的抑制作用;赤霉素对于芽胞萌发与生长有一定抑制作用.建立了蛇足石杉芽胞萌发培养体系,为进一步研究蛇足石杉生长发育、生理生化变化提供参考.

关键词: 蛇足石杉, 芽胞, 萌发, 培养基, 培养温度, 光照, 赤霉素

Abstract: The factors of influencing the gemmae germination of Huperzia serrata and growth condition are explored.The effect of culture medium,culture temperature,and light intensity and GA3 on the gemmae germination condition of Huperzia was studied,and the growth morphogenesis during the course of gemmae germination of Huperzia serrata was observed at the same time.Results showed that the best culture temperature for the gemmae germination was (22±2)℃,and the germination  rate  of the optimum  medium for Moore,the germination rate was as high as 82%,and the average plant length could be up to 14.620 mm.The second culture medium for the gemmae germination was Knudson C,the germination rate was 64%,and the average adventitious root length was about 5.158 mm.Darkness was conducive to gemmae germination,the germination rate was 92%,and the average adventitious root length was about 9.088 mm.Under 800lx weak light,the adventitious root grew slowly,and the adventitious root length could be up to 8.675 mm.1600lx light irradiation exhibited some inhibition for the adventitious root growth.And GA3  for gemmae germination and growth exhibited some inhibition. This study establishes the gemmae germination culture system for Huperzia serrata,which consitutes the foundation for further study on the growth,physiological and biochemical change of Huperzia serrata.

Key words: Huperzia serrata, gemmae, germination, culture medium, culture temperature, light intensity, GA3

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