journal6 ›› 2014, Vol. 35 ›› Issue (2): 85-89.DOI: 10.3969/j.issn.1007-2985.2014.02.019

• 生物资源 • 上一篇    下一篇

基于PsbA-trnH,RbcL和trnL-F序列的野生葡萄系统发育

唐云涛,粟桂蓉,谢毓,易浪波   

  1. (1.吉首大学生物资源与环境科学学院,湖南 吉首 416000;2.吉首大学植物资源保护与利用湖南省高校重点实验室,湖南 吉首 416000)
  • 出版日期:2014-03-25 发布日期:2014-05-09
  • 通讯作者: 易浪波(1980-),女,湖南娄底人,吉首大学生物资源与环境科学学院讲师,主要从事植物生态学研究.
  • 作者简介:唐云涛(1987-),男,湖南永州人,吉首大学生物资源与环境科学学院2011级生态学专业学生
  • 基金资助:

    湖南省教育厅科学研究重点资助项目(13A078)

Phylogenetic Analysis of Three Wild Grapes Based on PsbA-trnH,RbcL and trnL-F Sequences

 TANG  Yun-Tao, SU  Gui-Rong, XIE  Yu, YI  Lang-Bo   

  1. (1.College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China.2.Key Laboratory of Plant Resource Conservation and Utilization,Jishou University,Jishou 416000,Hunan China)
  • Online:2014-03-25 Published:2014-05-09

摘要:采用PCR技术从湘西地区的3种野生葡萄中分离PsbA-trnH、RbcL和trnL-F基因片段,克隆至pMD18-T载体中测序,结合GenBank/EMBL/DDBJ中公布的其他葡萄科植物相应cpDNA基因信息,使用Mega4构建系统发育进化树,分析其系统发育关系.结果显示,PsbA-trnH,RbcL和trnL-F这3种cpDNA基因序列均能用于葡萄科属间的分子鉴定,但结果与传统形态学分类存在一些差异,RbcL能用于葡萄属内种间的分子鉴定.

关键词: 野生葡萄, PsbA-trnH, RbcL, trnL-F, 系统发育

Abstract: PsbA-trnH,RbcL and trnL-F fragments were amplified from the three wild grapes in Xiangxi Autonomous Prefecture by PCR,and cloned into pMD18-T vector for sequencing.Based on PsbA-trnH,RbcL and trnL-F gene sequences,the phylogenetic trees between these three wild grapes and other related Vitaceae plants were constructed by software Mega4 for analyzing their phylogenetic relationship.The results showed that all of these three cpDNA fragments could be used as molecular markers in phylogenetic analysis among genera of Vitaceae,but of which results had a little difference from that of traditional morphological methods.Furthermore,RbcL gene fragment could be used for molecular identification among Vitis species.

Key words: wild grape, PsbA-trnH, RbcL, trnL-F, phylogeny

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