薰衣草内生真菌YDZ-8抗菌活性及菌种鉴定

doi:10.13438/j.cnki.jdzk.2023.02.007

吉首大学学报（自然科学版） ›› 2023, Vol. 44 ›› Issue (2): 47-51.DOI: 10.13438/j.cnki.jdzk.2023.02.007

薰衣草内生真菌YDZ-8抗菌活性及菌种鉴定

陈天意，努兰·拜都拉，玛依拉·吐尔德别克，恩特马克·布拉提白

（伊犁师范大学生物与地理科学学院，新疆伊宁 835000）

出版日期:2023-03-25 发布日期:2023-04-25
通讯作者: 恩特马克·布拉提白（1961—），男，新疆昭苏人，伊犁师范大学生物与地理科学学院教授，主要从事微生物分离、鉴定及活性成分研究.
作者简介:陈天意（1997—），男，甘肃兰州人，伊犁师范大学生物与地理科学学院硕士研究生，主要从事植物促生菌研究
基金资助:
国家自然科学基金资助项目（31960270）

Antibacterial Activity and Identification of Endophytic Fungi YDZ-8 Isolated from Lavandula angustifolia Mill.

CHEN Tianyi,BAIDOULA·Nulan,TUERDEBIEKE·Mayila,ENTOMACKY·Borrathyba

(College of Biology and Geography Sciences,Yili Normal University,Yining 835000,Xinjiang China)

Online:2023-03-25 Published:2023-04-25

摘要/Abstract

摘要：为了筛选具有抗菌活性的内生真菌，采用表面消毒法和组织分离法从药用植物薰衣草的根组织中分离内生真菌，用纸片法检测内生真菌的抗菌活性，通过形态特征观察和rDNA基因ITS序列分析方法对活性菌株进行鉴定.结果显示，从薰衣草的根中分离出9株形态特征有差异的内生真菌，其中菌株YDZ-8对金黄色葡萄球菌有较好的抑菌作用.根据菌株YDZ-8的形态特征和rDNA基因ITS序列同源性，鉴定该菌株为哈茨木霉菌.

关键词： 薰衣草, 内生真菌, 抗菌活性, ITS序列

Abstract: The endophytic fungi were isolated from the roots of Lavandula angustifolia Mill. by surface disinfection method and tissue isolation method,and the antibacterial activity against the Staphylococcus aureus was evaluated by paper disk diffusion method.The active isolates were identified in terms of their morphological features by phylogenetic analysis based on ITS sequence of rDNA gene.The results showed that the endophytic fungi of YDZ-8 had remarkable antibacterial activity against Staphylococcus aureus strain CGMCC1.872.Fungal morphological and molecular identification demonstrated that the endophytic fungi strain YDZ-8 represented Trichoderma harzianum.

Key words: Lavandula angustifolia Mill., endophytic fungi, antibacterial activity, ITS sequences

陈天意, 努兰·拜都拉, 玛依拉·吐尔德别克, 恩特马克·布拉提白. 薰衣草内生真菌YDZ-8抗菌活性及菌种鉴定[J]. 吉首大学学报（自然科学版）, 2023, 44(2): 47-51.

CHEN Tianyi, BAIDOULA·Nulan, TUERDEBIEKE·Mayila, ENTOMACKY·Borrathyba. Antibacterial Activity and Identification of Endophytic Fungi YDZ-8 Isolated from Lavandula angustifolia Mill.[J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(2): 47-51.

参考文献

[1] ANTONY STURZ,JERZY NOWAK.Endophytic Communities of Rhizobacteria and the Strategies Required to Create Yield Enhancing Associations with Corps[J].Applied Soil Ecology,2000,15(2):183-190.
[2] ANDERA STIERLE,GARY STROBLE,DONALD STIERLE,et al.Taxol and Taxane Production by Taxomyces Andreanae,an Endophytic Ungus of Pacific Yew[J].Science,1993,260(5105):214-216.
[3] 符继红,唐军,廖享,等.基于抑菌活性成分的薰衣草精油组效关系研究[J].质谱学报,2015,36(5):403-410.
[4] 姜黎,王向未,江英.薰衣草精油抑菌作用的研究[J].农产品加工,2011(4):73-75.
[5] 刘凡,李敏,顾爱星,等.薰衣草精油对几种植物病原菌的抑菌作用[J].新疆农业科学,2017,54(5):938-944.
[6] ALEXA ERSILIA,DANCIU CORINA,RADULOV ISIDORA,et al.Phytochemical Screening and Biological Activity of Mentha Piperita L. and Lavandula Angustifolia Mill. Extracts[J].Analytical Cellular Pathology,2018,2018:1-7.
[7] ZHAO Yunqi,CHEN Ran,WANG Yun,et al.In Vitro and in Vivo Efficacy Studies of Lavender Angustifolia Essential Oil and Its Active Constituents on the Proliferation of Human Prostate Cancer[J].Integrative Cancer Therapies,2017,16(2):215-226.
[8] MIASTKOWSKA MAGORZATA,KANTYKA TOMASZ,BIELECKA EWA,et al.Enhanced Biological Activity of a Novel Preparation of Lavandula Angustifolia Essential Oil[J].Molecules,2021,26(2458):21-40.
[9] BOGDAN MIHAELA,BUNGAU SIMONA,TIT DELIA,et al.Chemical Profile,Antioxidant Capacity,and Antimicrobial Activity of Essential Oils Extracted from Three Different Varieties (Moldoveanca 4,VisMagic 10,and Alba 7) of Lavandula Angustifolia[J].Molecules,2021,26(4381):20-39.
[10] MALEKIFARD FARNAZ,TAVASSOIL MOUSA,ALIMORADI MOHAMMAD.In Vitro Assessment of Anti-Trichomonas Effects of Zingiber Officinale and Lavandula Angustifolia Alcoholic Extracts on Trichomonas Gallinae[J].Veterinary Research Forum,2021,12(1):95-100.
[11] 熊天真,谢诗语,白蓉,等.薰衣草产酚酸内生真菌的分离及产物初步鉴定[J].食品与生物技术学报,2018,37(3):250-255.
[12] 曹宜,刘波,林营志,等.枯萎病尖孢镰刀菌的RAPD-PCR多态性分析[J].厦门大学学报,2004,8(43):74-78.
[13] 牛芳胜,马志强,毕秋艳,等.哈茨木霉菌与5种杀菌剂对番茄灰霉病菌的协同作用[J].农药学学报,2013,15(2):165-170.
[14] 宫占元,张国庆,于文莹,等. 哈茨木霉菌对水稻幼苗根际土壤微生物和酶活性的影响[J].干旱地区农业研究,2013,31(4):167-171.
[15] 马金慧,朱萍萍,茆振川,等.哈茨木霉菌株TRI2的鉴定及其对黄瓜根结线虫的防治作用[J].中国农学通报,2014,30(22):263-269.
[16] 关一鸣,潘晓曦,王莹,等.哈茨木霉菌、枯草芽孢杆菌对人参灰霉病和根腐病病原菌的拮抗作用[J].江苏农业科学,2014,42(5):123-125.
[17] 王雪,陈美兰,杨光,等.丛枝菌根真菌与哈茨木霉菌合用对连作丹参生长及质量的影响[J].中国中药杂志,2014,39(9):1574-1578.

薰衣草内生真菌YDZ-8抗菌活性及菌种鉴定

Antibacterial Activity and Identification of Endophytic Fungi YDZ-8 Isolated from Lavandula angustifolia Mill.

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