关键词： 溃疡性结肠炎, 甘草酸二铵, 淋巴细胞, 中性粒细胞, 凋亡

Abstract: To explore the effect of diammonium glycyrrhizinate on apoptosis of peripheral blood inflammatory cells in rats with ulcerative colitis.UC model was induced by TNBS and ethanol,both with colonic mucosa tissue,which were assigned into four groups,namely control group,model group,western medicine group (administrated by sulfasalazine enteric-coated tablets),and licorice group (administrated by diammonium glycyrrhizinate).Pathomorphological changes of colon tissue were dynamically observed in the 0,2^nd,and 4^th week after model establishment,and the apoptosis rates of LPL and PMN in the blood were dynamically observed by flow cytometry in the 2^nd and 4^th week respectively.Compared with the control group,pathological changes in colon tissue were showed in model group by HE staining,and the colon wet weight and the colon weight index increased.Compared with the model group,the pathological changes of colon tissue in DG group and western medicine group were recovered in a certain degree in the 2^nd and 4^th week.The colon wet weight and colon weight index decreased significantly in DG group in the 4^th week.Apoptosis of LPL and PMN in blood of UC model was delayed in the 2^nd week after model established,while DG and western medicine promoted poptosis of LPL and PMN.In the 4th week,apoptosis rate of LPL in UC model still decreased,but apoptosis rate of LPL in DG and western medicine group was higher than that of model group.It can be concluded that DG and sulfasalazine enteric coated tablets can still improve the apoptosis rate of LPL.Occurrence and development of ulcerative colitis is related with apoptosis reduction of LPL and PMN to some degree.DG can relieve UC  through regulating apoptosis rate of LPL and PMN in blood.

Key words: ulcerative colitis, diammonium glycyrrhizinate, lymphocytes, neutrophils, apoptosis