土木香内生细菌的分离鉴定及抑菌活性检测

doi:10.13438/j.cnki.jdzk.2019.04.011

吉首大学学报（自然科学版）

土木香内生细菌的分离鉴定及抑菌活性检测

吾鲁木汗·那孜尔别克，玛依拉·吐尔地别克，恩特马克·布拉提白

（伊犁师范大学生物与地理科学学院，新疆伊宁 835000）

出版日期:2019-07-25 发布日期:2019-07-31
通讯作者: 恩特马克·布拉提白（1961—），男，伊犁师范大学生物与地理科学学院教授，主要从微生物分类、鉴定及活性研究.E-mail:1632984092@qq.com.
基金资助:
伊犁师范大学微生物资源保护与利用重点实验室开放项目（YLUKNM201807）

Isolation of Endophytic Bacteria from Inula Helenium L. and Identification of Its Antibacterial Activity

NAZIERBIEKE Wulumuhan, TUERDEBIEKE Mayila, BORRATHYBAY Entomack

(College of Biology and Geography Sciences, Yili Normal University, Yining 835000, Xinjiang China)

Online:2019-07-25 Published:2019-07-31

摘要/Abstract

摘要：

利用组织分离法从用药植物土木香根、茎和叶中分离得到内生细菌32株.采用纸片法对菌株进行了抑菌活性研究，并运用生理生化与分子生物学方法进行种类鉴定.抑菌试验结果表明，10株内生菌发酵液对大肠杆菌、枯草芽孢杆菌以及禽多杀性巴氏杆菌均有不同程度的抑菌活性.鉴定结果表明，菌株YIR-5，YIR-7，YIR-8，YIR-9为假单胞菌属菌种，菌株YIR-3，YIR-6为沙雷氏菌，菌株YIR-4，YIS-1，YIS-2分别为水生拉恩氏菌、薄壁芽孢杆菌和蜡样芽孢杆菌，而菌株YIL-3归属于芽孢杆菌属.土木香内生细菌组成丰富，莫海威芽孢杆菌（Bacillus mojavensis）YIS-1对大肠杆菌、枯草芽孢杆菌和禽多杀性巴氏杆菌的抑菌活性最强.

关键词： 土木香, 内生细菌, 抑菌活性, 鉴定

Abstract:

Using the tissue separation method, 32 endophytic bacteria were isolated from the roots, stems and leaves of Inula helenium L., and antibacterial activiy of these strains were determined by using the agar disc diffusion test. Identification of these strains was determined by their morphological, physiological-biochemical properties and phylogenetic analysis based on 16S rRNA sequences. The results showed that the 10 endophytic bacteria had remarkable antibacterial activities to Escherichia coli, Bacillus subtilis and Pasteurella multocida. The endophytic strains YIR-5, YIR-7, YIR-8 and YIR-9 were classified as genus Pseudomonas, strains YIR-3 and YIR-6 as Serratia plymuthica, strains YIR-4, YIS-1 and YIS-2 as Rahnella aquatilis, Bacillus halotolerans and Bacillus cereus respectively, and the strain YIL-3 as the genus Bacillus. There are a variety of endophytic bacteria from Inula helenium L., and Bacillus mojavensis YIS-1 has the greatest antibacterial activity for escherichia coli, bacillus subtilis, and avian pasteurella multocida.

Key words: Inula helenium L., endophytic bacteria, antibacterial activity, identification

吾鲁木汗·那孜尔别克，玛依拉·吐尔地别克，恩特马克·布拉提白. 土木香内生细菌的分离鉴定及抑菌活性检测[J]. 吉首大学学报（自然科学版）, DOI: 10.13438/j.cnki.jdzk.2019.04.011.

NAZIERBIEKE Wulumuhan, TUERDEBIEKE Mayila, BORRATHYBAY Entomack. Isolation of Endophytic Bacteria from Inula Helenium L. and Identification of Its Antibacterial Activity[J]. Journal of Jishou University（Natural Sciences Edition), DOI: 10.13438/j.cnki.jdzk.2019.04.011.

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土木香内生细菌的分离鉴定及抑菌活性检测

Isolation of Endophytic Bacteria from Inula Helenium L. and Identification of Its Antibacterial Activity

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