微波辅助酶法水解草鱼鱼鳞的工艺条件

journal6 ›› 2010, Vol. 31 ›› Issue (2): 101-104.

微波辅助酶法水解草鱼鱼鳞的工艺条件

（吉首大学生物资源与环境科学学院，湖南吉首 416000)

出版日期:2010-03-25 发布日期:2012-04-18
通讯作者: 尹红(1964-),女,湖南洞口人, 吉首大学资源与环境科学学院高级实验师,主要从事食品检测技术与开发研究.
作者简介:曹光辉(1986-),男,湖南益阳人, 吉首大学资源与环境科学学院学生

Conditions of Microwave-Assisted hydrolization of Grass Carp Scale with Papain

（College of Biology and Environmental Sciences,Jishou University,Jishou 416000，Hunan China）

Online:2010-03-25 Published:2012-04-18

摘要/Abstract

摘要：研究微波萃取技术对木瓜蛋白酶水解草鱼鱼鳞条件的影响，探讨在一定的微波功率和辐射时间下，酶用量、底物浓度、酶解温度及酶解时间对水解度的影响，单因素试验确定较好的因素水平，正交试验确定最佳提取工艺条件.结果表明，在设定微波功率和辐射时间为400 W 60 s时，酶法水解草鱼鳞最佳工艺条件是：酶用量5 g/L、底物浓度20%、酶解温度60 ℃和酶解时间1 h.

关键词： 微波辅助, 酶法水解, 鱼鳞, 木瓜蛋白酶

Abstract: The impact of microwave extraction technique on papain hydrolyzing grass carp scale is investigated.Under certain conditions of microwave power and microwave radiation time,how enzyme dosage,substrate concentration,enzyme temperature and time influence degree of hydrolysis is discussed.The best extraction process conditions are determined by preferable level of factor which is decided by univariate test as well as orthogonal experiment.The results show that the best technological conditions of enzyme hydolyzing grass carp scale are:papain dosage,5 g/L,substrate concentration,20%,enzyme temperature,60 ℃,enzyme time,1 hour when microwave power is 400 W and microwave radiation time is 60 s.

Key words: Microwave-Assisted, enzyme hydrolization, scale, papain

曹光辉, 黄诚, 尹红, 李永平. 微波辅助酶法水解草鱼鱼鳞的工艺条件[J]. journal6, 2010, 31(2): 101-104.

CAO Guang-Hui, HUANG Cheng, YIN Hong, LI Yong-Ping. Conditions of Microwave-Assisted hydrolization of Grass Carp Scale with Papain[J]. journal6, 2010, 31(2): 101-104.

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