鸡蛋枣的组织培养与快速繁殖技术

journal6 ›› 2004, Vol. 25 ›› Issue (1): 26-28.

鸡蛋枣的组织培养与快速繁殖技术

( 1. 中国科学院华南植物研究所, 广东广州510650; 2. 中南林学院, 湖南株洲412006;3. 漳州师范学院生物系, 福建漳州363000)

出版日期:2004-03-15 发布日期:2012-11-06
作者简介:伍成厚(1968- ) , 男, 湖南邵阳人, 中国科学院华南植物研究所博士研究生, 主要从事植物组织培养的研究.
基金资助:
国家自然科学基金资助项目( 39670621)

Study on Fast Propagation of Juj ube C. V. Jidanzao by Tissue Culture

( 1. South China Institute of Botany, Chinese Academy of Sciences, GuangZhou 510650, China; 2. Central South Forestry University,Zhuzhou 412006, Hunan China; 3. Dept. of Biology, Zhangzhou Normal College, Zhangzhou, Fujian 363000, China)

Online:2004-03-15 Published:2012-11-06

摘要/Abstract

摘要：以鸡蛋枣的茎段为外植体, 进行了芽的诱导和成苗的研究. 结果表明: 在MS+ ZT1. 75 mg?L^-1 + KT 2. 0 mg?L^-1培养基, 茎段可以分化不定芽. 低浓度的NAA 促进不定芽的形成, 在MS + ZT 1. 75 mg?L^-1 + KT 2. 0 mg?L^-1+ NAA 0. 05 mg?L^-1中培养基芽的增殖系数达到4. 2 倍. 在1??2MS+ IBA 0. 8 mg?L^-1培养基中, 试管苗的生根率达到95. 0%. 试验还就外植体的消毒和组培苗的移植进行了探索.

关键词： 鸡蛋枣, 茎段, 不定芽, 组织培养, 快速繁殖

Abstract: The induct ion of adventit ious buds from stem segments and the forming of plantlets in vitro were studied in Jidanzao jujube .The results showed that adventitious buds could be induced from stem segments on the culture medium of MS+ ZT 1. 75 mg?L^-1+ KT 2. 0 mg?L^-1. The addition of low concentrations of NAA to the medium made higher induction rates of advent itious buds and 4. 2 times, the optimal proliferation number,was gotten on the medium of MS+ ZT 1. 75 mg?L^-1+ KT 2. 0 mg?L^-1+ NAA 0. 05 mg?L^-1. When the shoots formed in vitro got length of 3~ 4 cm, they were used for the induction of adventitious roots.The optimal medium for this purpose was 1??2MS+ IBA 0. 8 mg?L^-1and the forming rate of plantlets was over 95. 0%. In addition, the sterilization of explants and the transplant of plantlets were also studied.

Key words: Jidanzao jujube, stem segment, adventitious bud, tissue culture, fast propagation

伍成厚, 何业华, 谢碧霞, 胡芳名. 鸡蛋枣的组织培养与快速繁殖技术[J]. journal6, 2004, 25(1): 26-28.

WU Cheng-Hou, HE Ye-Hua, XIE Bi-Xia, HU Fang-Ming. Study on Fast Propagation of Juj ube C. V. Jidanzao by Tissue Culture[J]. journal6, 2004, 25(1): 26-28.

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