腐食酪螨和粉尘螨的共同抗原

journal6 ›› 2003, Vol. 24 ›› Issue (2): 31-34.

腐食酪螨和粉尘螨的共同抗原

（安徽理工大学医学院，安徽淮南232001）

出版日期:2003-06-15 发布日期:2012-11-07
作者简介:李朝品（1956-），男，安徽砀山人，医学博士，安徽理工大学医学院教授，主要从事病原生物学和免疫学的教学与科研工作.
基金资助:
安徽省自然科学基金重点资助项目（03043303）

Preliminary Study on Common Antigen of Tyrophagus Putrescentiae and Dermatophagoides Farine

(School of Medicine,Anhui University of Science & Technology,Huainan 232001，Anhui China)

Online:2003-06-15 Published:2012-11-07

摘要/Abstract

摘要：目的：探讨腐食酪螨和粉尘螨之间是否存在共同抗原.方法：对粉螨过敏患者进行腐食酪螨和粉尘螨特异性IgE相关性分析，用SDS-PAGE分析腐食酪螨和粉尘螨变应原，并对螨特异性IgE阳性血清进行免疫印迹试验及抑制试验.结果：42例粉螨过敏患者血清粉尘螨和腐食酪螨特异性IgE光密度值相关性较高（r=0.641 5，P＜0.01）.SDS-PAGE分析知腐食酪螨和粉尘螨变应原分子量均为10 kD～110 kD.免疫印迹抑制试验表明，腐食酪螨变应原可与粉尘螨变应原发生交叉反应.结论：腐食酪螨和粉尘螨间存在共同抗原.

关键词： 腐食酪螨, 粉尘螨, 共同抗原, 粉螨

Abstract: Objective To investigate whether there is common antigen between Tyrophagus putrescentiae (TP) and Dermatophagoides farine (DF) or not.Methods The correlation of ELISA optic densities between TP and DF-specific IgE in atopic sera was analyzed,and TP and DF extracts were analyzed with SDS-PAGE.IgE immunoblotting and IgE inhibition immunoblotting to TP and DF extracts with atopic sera were performed also.Results The ELISA optic densities of TP and DF-specific IgE in 42 atopic sera were correlated with each other (r=0.641 5，P＜0.01).By SDS-PAGE,the protein components of the TP and DF extracts were numerous protein bands between 10 kD and 110 kD.In addition,the presence of a cross-reactive IgE binding component in the TP and DF major allergens was investigated by IgE inhibition immunoblotting experiments.Conclusion There is common antigen between Tyrophagus putrescentiae and Dermatophagoides farine.

Key words: Tyrophagus putrescentiae, Dermatophagoides farine, common antigen, Acaridida

李朝品, 崔玉宝, 杨庆贵, 贺骥. 腐食酪螨和粉尘螨的共同抗原[J]. journal6, 2003, 24(2): 31-34.

LI Chao-Pin, CUI Yu-Bao, YANG Qing-Gui, HE Ji. Preliminary Study on Common Antigen of Tyrophagus Putrescentiae and Dermatophagoides Farine[J]. journal6, 2003, 24(2): 31-34.