丙肝患者PBMC mIL-2R及T细胞亚群检测

journal6 ›› 2001, Vol. 22 ›› Issue (4): 79-82.

丙肝患者PBMC mIL-2R及T细胞亚群检测

( 淮南工业学院分院,安徽淮南 232001)

出版日期:2001-12-15 发布日期:2013-01-05
作者简介:王克霞( 1956- ) ,女, 安徽省蚌埠市人,淮南工业学院分院副教授,主要从事传染病的教学和科研.

Detection of the Level of mIL- 2R and T Cells Subsets in PBMC in Patients With Hepatitis C

( School of Medicine, Huainan University of Technology, Huainan 232001, Anhui China)

Online:2001-12-15 Published:2013-01-05

摘要/Abstract

摘要：为了探讨丙型病毒性肝炎( 丙肝)患者膜白介素- 2 受体( mIL- 2R) 和 T 细胞亚群的表达水平及其在丙肝发病机理中的作用,用生物素- 链霉亲和素法对 203 例抗- HCV 阳性患者外周血单个核细胞进行 T 细胞亚群及植物血凝素( PHA) 诱导前后mIL- 2R 的检测. 总体结果显示丙肝患者外周血单个核细胞( PBMC) mIL- 2R 和 T 细胞亚群表达水平降低,与正常对照组相比,差异显著( P < 0. 01) .其中, 在静息状态和在 PHA 诱导状态,其 mIL- 2R 表达水平与正常对照组相比均低下( P < 0. 01) , 但急慢性丙肝患者中T 细胞亚群及 mIL- 2R 表达水平类似( P > 0. 05) . 急慢性丙肝患者与正常对照组相比,外周血 CD⁺₃ , CD⁺₄ 百分率降低, CD⁺₈ 百分率增高, CD⁺₄ / CD⁺₈ 比值下降( 从 P < 0. 05 降至 P< 0. 01) .说明丙肝患者体内存在明显的细胞免疫功能紊乱, T细胞活化障碍, 并与肝病的慢性化有关.

关键词： 丙型肝炎;膜白介素- 2受体( mIL- 2R);受体;T 细胞亚群, 外周血单个核细胞( PBMC)

Abstract: To study the level of membrane interleukin- 2 receptor ( mIL- 2R) and T cell subsets in PBMC in patients with hepatitis C and its role in the pathogenesis of hepatitis C, the level of mIL- 2R and T cells subsets in peripheral blood mononuclear cells ( PBMC) were detected with biotin- streptatividin technique before and after PHA induction in 203 cases of hepatitis C with positive HCV- RNA, ant i- HCV( + ) , ant i- HCV( - ) . The total results of the expressive level of mI L- 2R before and after PHA inducting and T cell subsets in PBMC were all lower in hepatitis C than those in normal controls ( P < 0. 01) .Among them, the levels of mI L- 2R were lower in silence than those in situation of PHA induction ( P < 0. 01) . The levels of CD⁺₃ , CD⁺₄ , CD⁺₄ / CD⁺₈ in acute and chronic hepatitis C with ant i- HCV( + ) were lower and CD⁺₈ was higher than those in normal controls ( P < 0. 01) .The function of cellular immunity, the levels of mI L- 2R and activation of T cells are obviously in disorder in patients with hepatitis C, and those were closely as sociated with changing of the hepatitis into chronic.

Key words: hepatitis C, membrane interleukin- 2 receptor, T- cell subsets, PBMC

王克霞, 李朝品, 王健. 丙肝患者PBMC mIL-2R及T细胞亚群检测[J]. journal6, 2001, 22(4): 79-82.

WANG Ke-Xia, LI Chao-Pin, WANG Jian. Detection of the Level of mIL- 2R and T Cells Subsets in PBMC in Patients With Hepatitis C[J]. journal6, 2001, 22(4): 79-82.

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