外源蛋白在巴氏毕赤酵母中高效表达的策略

journal6 ›› 2001, Vol. 22 ›› Issue (3): 40-44.

外源蛋白在巴氏毕赤酵母中高效表达的策略

(湖南师范大学生命科学院, 湖南长沙 410081)

出版日期:2001-09-15 发布日期:2013-01-05
作者简介:聂东宋( 1967- ) ,男, 湖南省衡阳县人,湖南师范大学硕士研究生,主要从事基因结构与功能研究.
基金资助:
国家自然科学基金资助项目( 39670392)

Optimpzed Strategies to Hyperexpress Heterologous Protein in Pichia Pastoris

( Life Science College, Hunan Normal University, Changsha 410081, Hunan China)

Online:2001-09-15 Published:2013-01-05

摘要/Abstract

摘要：高效表达外源蛋白, 在理论和实践上特别是在生物制药中具有重要意义, 巴氏毕赤酵母( Pichia pastoris) 是表达外源蛋白最理想的真核表达系统之一.影响外源蛋白在 P. pastoris中表达的因素很多, 主要包括外源基因自身的特性、载体、宿主细胞几个方面,了解和灵活运用它们的联系,有助于获得外源基因在 P. pastoris 中的高效表达.

关键词： 巴氏毕赤酵母, 外源蛋白, 高效表达

Abstract: It is very important to produce heterologous proteins both in theory and in pract ice, expecially for biopharmaceutical. Pichia pastoris has become one of the best eucaryotic expression systems in recent years.The factors influencing the high-level expression of heterologous protein in P. pastoris and the core strategies to hypere xpress heterologous proteins in P. pastoris were discussed in this paper.

Key words: pichia pastoris, heterologous protein, high-level expression

聂东宋, 梁宋平, 李敏. 外源蛋白在巴氏毕赤酵母中高效表达的策略[J]. journal6, 2001, 22(3): 40-44.

NIE Dong-Song, LIANG Song-Ping, LI Min. Optimpzed Strategies to Hyperexpress Heterologous Protein in Pichia Pastoris[J]. journal6, 2001, 22(3): 40-44.

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