高氧对新生小鼠视网膜谷氨酸释放的影响

doi:10.3969/j.issn.1007-2985.2014.02.020

journal6 ›› 2014, Vol. 35 ›› Issue (2): 90-93.DOI: 10.3969/j.issn.1007-2985.2014.02.020

高氧对新生小鼠视网膜谷氨酸释放的影响

彭振宇，高玲，彭湘萍

（1.中南大学湘雅二医院眼科，湖南长沙 410011；2.湘西自治州人民医院，吉首大学附属第一医院眼科，湖南吉首 416000；3.吉首大学医学院，湖南吉首 416000）

出版日期:2014-03-25 发布日期:2014-05-09
通讯作者: 高玲（1968-），女，湖南怀化人，中南大学湘雅二医院教授，博士生导师，主要从事眼疾病的发病机制与诊疗研究.
作者简介:彭振宇（1976-），湖南保靖人，中南大学湘雅二医院硕士生，湘西自治州人民医院副主任医师，主要从事玻璃体与视网膜疾病研究

Effectand Mechanism of Glutamate Release at Hyperxia Induced Retinal Injury in Newborn Mice

PENG Zhen-Yu, GAO Ling, PENG Xiang-Ping

（1.Department of Ophthalmology，the Second Xiangya Hospital of Central South University，Changsha 410011，China;2.Department of Ophthalmology，the People’s Hospital of Xiangxi Autonomous Prefecture，Jishou 416000，Hunan China;3.Medical College of Jishou University,Jishou 416000,Hunan China)

Online:2014-03-25 Published:2014-05-09

摘要/Abstract

摘要：为研究新生小鼠高氧状态下视网膜谷氨酸摩尔质量浓度的变化及其机制，探讨谷氨酸在高氧视网膜损伤中的作用，实验组将新生小鼠置于体积分数为95%的高氧环境中造成视网膜损伤，对照组将新生小鼠置于正常空气中，HE染色观察视网膜损伤情况，多功能酶标仪检测视网膜谷氨酸(Glu)摩尔质量浓度变化，逆转录聚合酶链反应(RT-PCR)检测视网膜谷氨酸转运体(GLAST)与谷氨酰胺合成酶(GS)mRNA表达情况.结果发现，与空气对照组比较，高氧处理组视网膜受损，视网膜Glu摩尔质量浓度增加，GLAST和GS mRNA表达下降（P<0.05）.高氧可导致新生小鼠视网膜的谷氨酸增高，其机制与GLAST和GS mRNA表达下降有关.谷氨酸增加可能是高氧导致视网膜损伤的原因之一.

关键词： 高氧, 谷氨酸, 视网膜, 新生, 小鼠

Abstract: The concentration change and mechanism of glutamate at hyperxia induced retinal injury in newborn mice retinal injury are explored.The experimental group was put in 95% hyperxia environment to induce retinal damage,and the control groups in normal air.HE staining was used to observe retinal injury,ELIASA to detecte the glutamate (Glu) concentration,and reverse transcription polymerase chain reaction(RT-PCR) to analyze the expression of retinal glutamate transporter (GLAST) and glutamine synthetase (GS) mRNA.The results showed that,compared with control group,hyperxia treatment group with retinal injury showed the increased Glucontent,and decreased GLAST and GS mRNA expression.It thus can be concluded that hyperxia can lead to the increase of retinal glutamate in newborn mice,the mechanism of which was associated with decreased GLAST and GS mRNA expression.Glutamate increase may be one of the reasons for the hyperxia induced retinal injury.

Key words: hyperxia, glutamate, retina；newborn, mice

彭振宇, 高玲, 彭湘萍. 高氧对新生小鼠视网膜谷氨酸释放的影响[J]. journal6, 2014, 35(2): 90-93.

PENG Zhen-Yu, GAO Ling, PENG Xiang-Ping. Effectand Mechanism of Glutamate Release at Hyperxia Induced Retinal Injury in Newborn Mice[J]. journal6, 2014, 35(2): 90-93.

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高氧对新生小鼠视网膜谷氨酸释放的影响

Effectand Mechanism of Glutamate Release at Hyperxia Induced Retinal Injury in Newborn Mice

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