莱菔子油抗冻伤作用及机制探索

doi:10.13438/j.cnki.jdzk.2025.05.009

吉首大学学报（自然科学版） ›› 2025, Vol. 46 ›› Issue (5): 59-68.DOI: 10.13438/j.cnki.jdzk.2025.05.009

莱菔子油抗冻伤作用及机制探索

雷艳，靳硕，狄开银，刘昱卉，杨廷，肖雅，徐达，吴光辉

（1.湘西土家族苗族自治州农业科学研究院，湖南吉首 416000；2.吉首大学药学院，湖南吉首 416000；3.武陵山区药用资源化学与药理湖南省高校重点实验室，湖南吉首 416000）

出版日期:2025-09-25 发布日期:2025-11-07
作者简介:雷艳（1988—），女，湖南临武人，湘西土家族苗族自治州农业科学研究院高级农艺师，硕士，主要从事蔬菜生理功能研究
基金资助:
湘西自治州农科院青年科技创新基金项目（XXNK202440）；湖南省现代农业中药材产业技术体系项目（湘农办发[2024]46号）

Study on the Effect and Mechanism of Semen Raphani Oil in the Treatment of Chilblains

LEI Yan,JIN Shuo,DI Kaiyin,LIU Yuhui,YANG Ting,XIAO Ya,XU Da,WU Guanghui

(1.Agricultural Scientific Research Institute of Xiangxi Tujia and Miao Autonomous Prefecture,Jishou 416000,Hunan China;2.School of Pharmacy,Jishou University,Jishou 416000,Hunan China;3.Key Lab on Chemistry and Pharmacology of Medicinal Resources in Wulingshan (College of Hunan Province),Jishou University,Jishou 416000,Hunan China)

Online:2025-09-25 Published:2025-11-07

摘要/Abstract

摘要：为了探讨莱菔子油抗冻伤的作用及机制，通过建立小鼠冻伤模型，观察莱菔子油对小鼠足肿胀率、疼痛阈值和冻伤组织病理变化的影响，并利用网络药理学技术对莱菔子油中的抗冻伤成分、靶点和信号通路进行了分析.动物实验结果表明，莱菔子油可明显缓解小鼠足肿胀，提高冻伤小鼠疼痛阈值，缓解皮肤水肿，减少冻伤组织中肥大细胞数目.网络药理学预测结果表明，莱菔子油治疗冻伤存在38个潜在靶点，MAOB，HSP90AB1，PTGS2，PTGS1，ACTB，MYC，PGR，NCOA2，RELA和RXRA靶点为莱菔子油治疗冻伤的核心靶点，主要涉及的信号通路包括神经活性配体信号、化学致癌作用-受体激活、人巨细胞病毒感染和磷脂酰肌醇-3 -激酶-蛋白激酶B信号通路等，主要涉及的生物功能包括平滑肌收缩、异生物质刺激反应和产热反应等.

关键词： 莱菔子油, 冻伤, 小鼠, 作用机制

Abstract: To investigate the anti-chilblain effect and mechanism of Semen Raphani oil,a mouse chilblains model was established to observe the effects of Semen Raphani oil on paw swelling rate,pain threshold,and pathological changes in chilblained tissues.Network pharmacology was used to explore the anti-chilblain components,targets,and signaling pathways of Semen Raphani oil.Animal experiments showed that Semen Raphani oil significantly alleviated paw swelling,increased the pain threshold in chilblained mice,relieved skin edema,and reduced the number of mast cells in chilblained tissues.Network pharmacology predictions indicate that Semen Raphani oil may interact with 38 potential targets in the treatment of chilblains.The core targets include MAOB,HSP90AB1,PTGS2,PTGS1,ACTB,MYC,PGR,NCOA2,RELA,and RXRA.The primary signaling pathways involved are neuroactive ligand signaling,chemical carcinogenesis-receptor activation,human cytomegalovirus infection,and the phosphatidylinositol-3-kinase-protein kinase B (PI3K-Akt) pathway.Key biological functions associated with these targets include smooth muscle contraction,xenobiotic stimulus response,and thermogenesis.

Key words: semen raphani oil, chilblains, mouse, mechanism

雷艳, 靳硕, 狄开银, 刘昱卉, 杨廷, 肖雅, 徐达, 吴光辉. 莱菔子油抗冻伤作用及机制探索[J]. 吉首大学学报（自然科学版）, 2025, 46(5): 59-68.

LEI Yan, JIN Shuo, DI Kaiyin, LIU Yuhui, YANG Ting, XIAO Ya, XU Da, WU Guanghui. Study on the Effect and Mechanism of Semen Raphani Oil in the Treatment of Chilblains[J]. Journal of Jishou University(Natural Sciences Edition), 2025, 46(5): 59-68.

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莱菔子油抗冻伤作用及机制探索

Study on the Effect and Mechanism of Semen Raphani Oil in the Treatment of Chilblains

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