水杨酸对烟草GRPs基因表达的影响

吉首大学学报（自然科学版） ›› 2008, Vol. 29 ›› Issue (2): 86-91.

水杨酸对烟草GRPs基因表达的影响

(1.四川大学生命科学学院，四川成都610064；2.云南省烟草科学研究所，云南玉溪 653100；3.云南农业大学食品学院，云南昆明650201)

出版日期:2008-03-25 发布日期:2012-05-25
作者简介:董霞（1965-），四川崇州人，四川大学生命科学学院教授，主要从事植物分子生物学研究
基金资助:
国家自然科学基金资助项目(30560062)

The Effect of Salicylic Acid on Expression of the Gene Coding Glycine-Rich Protein Binding RNA in Tobacco

(1.College of Life Science,Sichuan University,Chengdu 610064，China；2.Yunnan Tobacco Science Research Institute,Yuxi 653100，Yunnan China;3.Faculty of Food Science,Yunnan Agricultural University,Kunming 650201，China)

Online:2008-03-25 Published:2012-05-25

摘要/Abstract

摘要：通过RT-PCR得到富含甘氨酸RNA结合蛋白（GRPs）基因片段，利用northern杂交技术对水杨酸诱导不同时间不同烟草GRPs基因的表达模式进行研究.结果表明：水杨酸能诱导烟草GRPs基因上调表达，3 h达到最高，然后开始下降；但3个TMV抗性不同的供试烟草增加幅度不同，基因表达幅度与抗性有一定关系,抗性强的红花大金元表达增幅大于抗性较弱的云烟85.本研究明确了水杨酸诱导下烟草该基因的表达谱，揭示了SA诱导烟草不同抗性的品种的幅度不同，并且发现诱导后3 h为关键点，表明GRPs基因可作为烟草水杨酸代谢途径中的一个标记基因研究其途径对逆境的应答.

关键词： 水杨酸, 富含甘氨酸RNA结合蛋白基因（GRP）, northern杂交, 基因表达, 烟草

Abstract: The gene fragments(GRPs) coding glycine-rich protein binding RNA in tobacco materials were got by RT-PCR.Northern hybridization blot analysis was used to demonstrate the expression pattern of the GRP gene at different times after treatment of salicylic acid in different tobacco materials.Inducded by salicylic acid,the GRP genes of Nicotiana could up-regulate expression.The expression attained the highest in 3h,then began descending.The three tobacco varieties had different resistance abilities to TMV.The expression level of two genes correlated with the resistance ability.The increase of two genes in Honghuadajingyuan with stronger resistance ability was higher than that of Yunyan 85 with less resistance ability.The expression pattern of the gene induced by salicylic acid in tobacco was got.It was found that in different tobacco varieties with different resistance abilities,the up-regulation extents of expression of the GRP gene were quite different after treatment of salicylic acid.And it is also found that the up expression became significant after 3 hours treatment.From the results of this study,it is suggested that GRP gene can be used as a marker gene to investigate salicylic acid metabolism in tobacco varieties so as to demonstrate their response to abiotic stresses.

Key words: salicylic acid；the gene coding glycine-rich protein binding RNA, northern hybridization, expression pattern, tobacco

董霞, 李文正, 黄夸克, 兰建强, 李正风, 刘世贵. 水杨酸对烟草GRPs基因表达的影响[J]. 吉首大学学报（自然科学版）, 2008, 29(2): 86-91.

DONG Xia, LI Wen-Zheng, HUANG Kua-Ke, LAN Jian-Qiang, LI Zheng-Feng, LIU Shi-Gui. The Effect of Salicylic Acid on Expression of the Gene Coding Glycine-Rich Protein Binding RNA in Tobacco[J]. Journal of Jishou University(Natural Sciences Edition), 2008, 29(2): 86-91.

参考文献

[1] HIROSE T,SUGITA M,SUGIURA M.cDNA Structure,Expression and Nucleic Acid-Binding Properties of Three RNA-Binding Proteins in Tobacco:Occurrence of Tissue-Specific Alternative Splicing [J].Nucleic Acids Res,1993,21:3 981-3 987.

[2] HIROSE T,SUGITA M,SUGIURA M.Characterization of a cDNA Encoding a Novel Type of RNA-Binding Protein in Tobacco:Its Expression and Nucleic Acid-Binding Properties [J].Mol.Gen.Genet.，1994,244 (4):360-366.

[3] MORIGUCHI K,SUGITA M,SUGIURA M.Structure and Subcellular Localization of a Small RNA-Binding Protein from Tobacco [J].Plant J,1997,12 (1):215-221.

[4] SMART L B,CAMERON K D,BENEETT A B.Isolation of Genes Predominantly Expressed in Guard Cells and Epidermal Cells of Nicotiana Glauca [J].Plant Mol Biol,2000,42(6):857-69.

[5] YE Z H,VARNER J E.Tissue-Specific Expression of Cell Wall Proteins in Developing Soybean Tissues [J].Plant Cell,1991,3 (1):23-37.

[6] CONDIT C M．Developmental Expression and Localization of Petunia Glycine-Rich Protein [J].Plant Cell,1993,5(3):277-288.

[7] SACHETTO-MARTINS G,FRANCO L,DE OLIVEIRA D.Plant Glycine-Rich Proteins:a Family or Just Proteins with a Common Motif [J]. Bioch Biophys Acta,2000,1492(1):1-14.

[8] NOMATA T,KABEYA Y,SATO N.Cloning and Characterization of Glycine-Rich RNA-Binding Protein cDNAs in the Moss Physcomitrella Patens [J].Plant Cell Physio,2004,45(1):48-56.

[9] DE OLIVEIRA D E,SEURINCK J,INZE D,et al.Differential Expression of Five Arabidopsis Genes Encoding Glycine-Rich Proteins [J].Plant Cell,1990,2(5):427-436.

[10] KUC J.Induced Immunity to Plant Disease [J].BioScience,1982,32(11):854-860.

[11] DELANEY T P,UKNES S,VERNOOIJ B,et al. A central Role of Salicylic Acid in Plant Disease Resistance [J].Science,1994,266:1 247-1 250.

[12] 李国婧,周燮.水杨酸与植物抗非生物胁迫 [J].植物学通报,2001,18(3):295-302.

[13] FUSARO AF,BOCCA SN,RAMOS RL,et al.AtGRP2,a Cold-Induced Nucleo-Cytoplasmic RNA-Binding Protein,has a Role in Flower and Seed Development [J].Planta,2007,225(6):1 339-1 351.

[14] 于放,张冬艳,白凤武，等.水杨酸对喜树细胞次级代谢的诱导作用 [J].高技术通讯,2005,15（1）：70-73.

[15] 刘宗旨，王建龙，王群等.水稻Osgrp-2基因的结构、表达特性和染色体定位 [J].中国科学C辑,2003,33（3）:215-223.