关键词： AFLP, 鲤, 生态安全

Abstract: The genome of first filial generation,whose parents were carp with different rate of transferred Oncorhunchus Suckley growth hormone gene,were scanned to estimate its eco-security,using the wild carp of Fu-yuan,common carp of Songhua River of Harbin and cultural carp as control.Control group,1% experimental group,10% experimental group,25% experimental group,50% experimental group,60% experimental group,H group and Y group were amplified with 10 pairs of AFLP primers.663 locus were obtained.The numbers of poly-bonds locus were all more than 50% in every population.With the rate of transgenic parents increasing,the numbers of filial generation polymorphism locus  descended.Number of alleles (Na),effective number of alleles (Ne),Nei’s gene diversity (H),Shannon’s Information index (I),genetic inter-group similarity  (S),genetic distance(D),the coefficient of gene differentiation among populations within species (Gst) and gene flow (Nm) were calculated with PopGen32 software.Ten groups were compared.The results showed that the largest value of Na,Ne,Nei’s gene diversity index (H) and Shannon information index (I) were all in Y group,and the second,in the control groups.The minimum were in the H group.In five filial generation experimental groups of  transgenic parents of different rate,with the rate of trans-gene parents increasing,all the four indexes tended to descend.Genetic  inter-group similarity (S) and genetic distance (D) showed that 25%,50% and 60% experimental group were separated from other groups in different degree.Especially,the 60% experimental group was dramatically far away from the same group.It is thus evident that 60% experimental group had great difference with the others.The comparing result of the five trans-gene experimental groups,H group and Y group Gst and gene flow showed that gene differentiation was larger and gene flow was small.With the rate of trans-gene parents increasing,the Gst of filial generation increased and the gene flow  descended.From those,it can be seen that the genome of the first filial generation changed in a certain regularity with the increase of parents transgenic rate.All of these illuminate that more transgenic fish escape,more influence to the wildlife population.Transgenic fish should be prevented from escaping to reduce the genome pollution of wildlife population.

Key words: AFLP, Cyprinus carpio, ecological safety