利用WGCNA挖掘黄精属植物多糖合成通路关键基因

doi:10.13438/j.cnki.jdzk.2023.05.009

吉首大学学报（自然科学版） ›› 2023, Vol. 44 ›› Issue (5): 64-71.DOI: 10.13438/j.cnki.jdzk.2023.05.009

利用WGCNA挖掘黄精属植物多糖合成通路关键基因

汪蓉，孟然，陈雪梅，孟盈

（吉首大学生物资源与环境科学学院，湖南吉首 416000）

出版日期:2023-09-25 发布日期:2023-11-21
通讯作者: 孟盈（1973—），女，云南宣威人，吉首大学生物资源与环境科学学院教授，博士，主要从事植物系统演化研究.E-mail:mengying@jsu.edu.cn.
基金资助:
国家自然科学基金资助项目（32160052）；吉首大学大学生创新创业训练计划项目（201910531019）

Key Genes for Polysaccharide Synthesis Pathway from Polygonatum Based on WGCNA

WANG Rong,MENG Ran,CHEN Xuemei,MENG Ying

(College of Biological Resources and Environmental Sciences,Jishou University,Jishou 416000,Hunan China)

Online:2023-09-25 Published:2023-11-21

摘要/Abstract

摘要：为了获取黄精属植物多糖类有效成分合成的关键基因，基于转录组差异表达数据，采用加权共表达网络分析方法，并结合KEGG数据库中多糖合成通路信息，计算核心基因在多花黄精和玉竹的叶片及根状茎之间表达模式的相关性.结果表明，FBA（Fructose-Bisphosphate Aldolase）基因和GOLS（Galactinol Synthase）基因在叶片和根状茎之间对应多个转录本的表达量，在皮尔森线性回归计算结果中显示了强相关性，是黄精属植物多糖活性成分合成通路的关键基因.

关键词： 加权共表达网络, 黄精属, 多糖合成通路, 转录组

Abstract: In order to find possible key genes related to the synthesis of polysaccharides in Polygonatum,this study explored the correlation between the expression patterns of core genes in leaves and rhizomes of Polygonatum cyrtonema Hua and Polygonatum odoratum (Mill.) Druce in combination with polysaccharide synthesis pathways from KEGG database using transcriptome data with expression difference and weighted gene co-expression network analysis (WGCNA).Our results indicated that FBA (fructose-bisphosphate aldolase) and GOLS (galactinol synthase) showed a strong correlation in Pearson linear regression calculation,which demonstrated that they are possible key genes for polysaccharide synthesis pathway in Polygonatum.

Key words: WGCNA, Polygonatum, polysaccharide synthesis pathway, transcriptome

汪蓉, 孟然, 陈雪梅, 孟盈. 利用WGCNA挖掘黄精属植物多糖合成通路关键基因[J]. 吉首大学学报（自然科学版）, 2023, 44(5): 64-71.

WANG Rong, MENG Ran, CHEN Xuemei, MENG Ying. Key Genes for Polysaccharide Synthesis Pathway from Polygonatum Based on WGCNA[J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(5): 64-71.

参考文献

[1] MENG Ran,LUO Liying,ZHANG Jiyuan,et al.The Deep Evolutionary Relationships of the Morphologically Heterogeneous Nolinoideae (Asparagaceae) Revealed by Transcriptome Data[J].Frontiers in Plant Science,2021,11:1-12.
[2] 王天梅,陈松树,李丹丹,等.不同产地黄精主要成分比较研究[J].特产研究,2021,43(4):44-48.
[3] 焦劼.黄精种质资源研究[D].咸阳:西北农林科技大学,2018.
[4] 王庆.药用植物黄精鉴别研究进展[J].现代园艺,2020,43(13):35-37.
[5] 宁露云,李芷仪,李志远,等.多花黄精繁殖技术与分子生物学研究进展[J].分子植物育种,2022,6(6):1-21.
[6] 刘跃钧,曾岳明,叶征莺,等.多花黄精栽培技术研究进展[J].中国现代中药,2022,24(4):715-720.
[7] 杨迎,侯婷婷,王威,等.黄精多糖的药理作用研究进展[J].现代药物与临床,2022,37(3):659-665.
[8] 黄家鹏,赵健,赵璇,等.黄精防治阿尔茨海默病相关认知功能障碍的机制研究进展[J].医学综述,2022,28(8):1606-1612.
[9] 吴倩,陈文明,陈柱梁,等.黄精干预糖尿病及其并发症的研究进展[J].中医药导报,2022,28(4):180-185.
[10] 何沛煜,张军银,赵永艳,等.黄精药用价值及保健食品应用研究进展[J].海峡药学,2021,33(12):31-35.
[11] 石乃星,文国松,赵明富.黄精属植物DNA分子鉴定技术应用研究进展[J].植物遗传资源学报,2021,22(5):1209-1218.
[12] 赖隽晖,李秀霞.药用植物玉竹药理作用研究进展[J].黑龙江农业科学,2021,2:132-135.
[13] 王宇,谭福燕,张利萍,等.黄精转录组特性分析及相关功能基因研究[J].四川师范大学学报(自然科学版),2022,45(1):103-109.
[14] 叶碧欢,杨阳,朱杰丽,等.基于比较转录组学的多花黄精黄酮类化合物合成基因表达分析[J].食品与生物技术学报,2022,41(4):84-92.
[15] WANG Shiqiang,WANG Bin,HUA Wenping,et al.De Novo Assembly and Analysis of Polygonatum sibiricum Transcriptome and Identification of Genes Involved in Polysaccharide Biosynthesis[J].International Journal of Molecular Sciences,2017,18(9):1-17.
[16] 肖韵铮,韩世明,秦昭,等.滇黄精转录组测序及类黄酮合成相关基因的分析[J].河南农业大学学报,2020,54(6):931-940.
[17] 李达.茶树细胞色素CYP82Ds基因克隆及其功能验证[D].杭州:浙江大学,2021.
[18] 郭永春,王鹏杰,金珊,等.基于WGCNA鉴定茶树响应草甘膦相关的基因共表达模块[J].中国农业科学,2022,55(1):152-166.
[19] 赵丹,熊燕,华永琳,等.牦牛UCP1基因生物学特性及组织表达分析[J].西南民族大学学报(自然科学版),2021,47(2):139-148.
[20] WANG Chenkai,PENG Daiyin,ZHU Jinhan,et al.Transcriptome Analysis of Polygonatum cyrtonema Hua:Identification of Genes Involved in Polysaccharide Biosynthesis[J].Plant Methods,2019,15(1):1-14.
[21] NI Xianzhi,JIN Chenzhong,LIU Aiyu,et al.Physiological and Transcriptomic Analyses to Reveal Underlying Phenolic Acid Action in Consecutive Monoculture Problem of Polygonatum odoratum[J].BMC Plant Biology,2021,21(1):1-13.
[22] 陈蕾,万婉,刘艳,等.黄精有效成分提取测定的研究[J].赤峰学院学报(自然科学版),2019,35(9):37-39.
[23] 吴丰鹏.九蒸九制对威海黄精有效成分的影响研究[D].哈尔滨:哈尔滨工业大学,2020.
[24] 董垚垚,周涛,乔玉双,等.泰山黄精的研究进展[J].中国果菜,2022,42(2):21-28.
[25] SCHNARRENBERGER CLAUS,KRGER INGO.Distinction Between Cytosol and Chloroplast Fructose-Bisphosphate Aldolases from Pea,Wheat,and Corn Leaves[J].Plant Physiology,1986,80(2):301-304.
[26] CARRERA DNIEL RPD,GEORGE GAVIN M,FISCHER-STETTLER MICHAELA,et al.Distinct Plastid Fructose Bisphosphate Aldolases Function in Photosynthetic and Non-Photosynthetic Metabolism in Arabidopsis[J].Journal of Experimental Botany,2021,72(10):3739-3755.
[27] QAISAR UZMA,IRFAN MUHAMMAD,MEQBOOL ASMA,et al.Identification,Sequencing and Characterization of a Stress Induced Homologue of Fructose Bisphosphate Aldolase from Cotton[J].Canadian Journal of Plant Science,2010,90(1):41-48.
[28] MUKHERJEE SRITAMA,SENGUPTA SONALI,MUKHERJEE ABHISHEK,et al.Abiotic Stress Regulates Expression of Galactinol Synthase Genes Post-Transcriptionally Through Intron Retention in Rice[J].Planta,2019,249(3):891-912.
[29] DOS SANTOS TIAGO BENEDITO,VIEIRA LUIZ GONZAGA ESTEVES.Involvement of the Galactinol synthase Gene in Abiotic and Biotic Stress Responses:A Review on Current Knowledge[J].Plant Gene,2020,24:1-8.

利用WGCNA挖掘黄精属植物多糖合成通路关键基因

Key Genes for Polysaccharide Synthesis Pathway from Polygonatum Based on WGCNA

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价