吉首大学学报(自然科学版) ›› 2023, Vol. 44 ›› Issue (5): 64-71.DOI: 10.13438/j.cnki.jdzk.2023.05.009

• 生物·化学 • 上一篇    下一篇

利用WGCNA挖掘黄精属植物多糖合成通路关键基因

汪蓉,孟然,陈雪梅,孟盈   

  1. (吉首大学生物资源与环境科学学院,湖南 吉首 416000)
  • 出版日期:2023-09-25 发布日期:2023-11-21
  • 通讯作者: 孟盈(1973—),女,云南宣威人,吉首大学生物资源与环境科学学院教授,博士,主要从事植物系统演化研究.E-mail:mengying@jsu.edu.cn.
  • 基金资助:
    国家自然科学基金资助项目(32160052);吉首大学大学生创新创业训练计划项目(201910531019)

Key Genes for Polysaccharide Synthesis Pathway from Polygonatum Based on WGCNA

WANG Rong,MENG Ran,CHEN Xuemei,MENG Ying   

  1. (College of Biological Resources and Environmental Sciences,Jishou University,Jishou 416000,Hunan China)
  • Online:2023-09-25 Published:2023-11-21

摘要:为了获取黄精属植物多糖类有效成分合成的关键基因,基于转录组差异表达数据,采用加权共表达网络分析方法,并结合KEGG数据库中多糖合成通路信息,计算核心基因在多花黄精和玉竹的叶片及根状茎之间表达模式的相关性.结果表明,FBA(Fructose-Bisphosphate Aldolase)基因和GOLS(Galactinol Synthase)基因在叶片和根状茎之间对应多个转录本的表达量,在皮尔森线性回归计算结果中显示了强相关性,是黄精属植物多糖活性成分合成通路的关键基因.

关键词: 加权共表达网络, 黄精属, 多糖合成通路, 转录组

Abstract: In order to find possible key genes related to the synthesis of polysaccharides in Polygonatum,this study explored the correlation between the expression patterns of core genes in leaves and rhizomes of Polygonatum cyrtonema Hua and Polygonatum odoratum (Mill.) Druce in combination with polysaccharide synthesis pathways from KEGG database using transcriptome data with expression difference and weighted gene co-expression network analysis (WGCNA).Our results indicated that FBA (fructose-bisphosphate aldolase) and GOLS (galactinol synthase) showed a strong correlation in Pearson linear regression calculation,which demonstrated that they are possible key genes for polysaccharide synthesis pathway in Polygonatum.

Key words: WGCNA, Polygonatum, polysaccharide synthesis pathway, transcriptome

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