吉首大学学报(自然科学版)

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Trichoderma Reesei菌生物降解酶CIP 1和CIP 2的生物信息学分析

张红,魏雪莹,陈加蓓,陈思佳,贺建武   

  1. (1.吉首大学生物资源与环境科学学院,湖南 吉首 416000;2.吉首大学杜仲综合利用技术国家地方联合工程实验室,湖南 吉首 416000)
  • 出版日期:2018-11-25 发布日期:2018-12-07
  • 通讯作者: 贺建武(1985—),男(土家族),吉首大学生物资源与环境科学学院实验师,主要从事药用资源与生态学研究.E-mail:hejsu@jsu.edu.cn.
  • 基金资助:

    吉首大学校级科研项目(JD16007,JDX16018,JDX16019)

Bioinformatics Analysis of Trichoderma Reesei  Biodegrading Enzyme CIP 1 and CIP 2

ZHANG Hong,WEI Xueying,CHEN Jiabei,CHEN Sijia,HE Jianwu   

  1. (1.College of Biological Resources and Environmental Science,Jishou University,Jishou 416000,Hunan China;2.National Joint Engineering Lab. for Eucommia Utilization,Jishou University,Jishou 416000,Hunan China)
  • Online:2018-11-25 Published:2018-12-07

摘要:

运用生物信息学方法,对已报道的Trichoderma reesei QM6a菌株生物降解酶CIP 1和CIP 2基因进行了生物信息学分析,以明确里氏木霉生物降解酶CIP 1和CIP 2的理化性质、二级结构、信号肽、定位、跨膜结构、磷酸化位点及进化关系.结果表明:CIP 1和CIP 2均属于稳定蛋白,含有大量无规则卷曲;信号肽剪切位点分别在19~20和17~18位的氨基酸之间;两者无螺旋卷曲结构,无跨膜结构域,并定位在分泌途径信号肽(SP)上.

关键词: 里氏木霉, 生物信息学, 纤维素, 生物降解

Abstract:

Bioinformatics analysis was performed on the CIP 1 and CIP 2 genes of biodegradation enzymes of the reported strain Trichoderma reesei QM6a through bioinformatics methods.The physical and chemical properties,secondary structure,signal peptide,localization,transmembrane structure,phosphorylation sites and evolutionary relationships of Trichoderma reesei biodegradable enzyme CIP 1 and CIP 2 were identified.The results showed that CIP 1 and CIP 2 are stable protein,containing a large number of random curls.Signal peptide cleavage sites are between amino acids 19~20,17~18,respectively.Both have no helical crimp structure,no transmembrane domain,and are located on the secretory pathway signal peptide (SP).

Key words: Trichoderma reesei, bioinformatics, cellulose, biodegradation

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