journal6 ›› 2000, Vol. 21 ›› Issue (3): 11-13.
• 重点学科 • 上一篇 下一篇
出版日期:
发布日期:
作者简介:
Online:
Published:
摘要:针对芦荟自然繁殖率低下的情况, 探讨了利用组织培养方法解决生产中繁殖的问题.在叶和茎 2 种外植体中,嫩茎的出愈率最高,是理想的快速繁殖材料,较适宜的诱导愈伤组织的激素组合是 BA 2. 0 mg•L- 1+ IBA 0. 2 mg•L- 1, 诱导不定芽的适宜激素组合是 BA 3. 0 mg•L- 1+ NAA 1. 0 mg•L- 1, 而根的诱导则是在1/ 2MS+NAA 1. 0 mg•L- 1+ 庶糖 1. 5%+活性碳 3%培养基上进行.
关键词: 芦荟, 组织培养, 愈伤组织, 不定芽
Abstract: Because the fruiting rate and germinative percentage of Aloe vera L. are lower, tissue culture was explored to solve the propagating problem in the production. The results show that the bud is the best material in speeding propagation among the two explants ( stem, leaf ) as it has the biggest inductivity, the best growth and it is easy to be in ducted adventitious buds. The suitable phytohormone compositions to induct callus, adventitious buds and roots are respectively BA 2. 0 mg•L- 1+ IBA 0. 2 mg•L- 1, BA 3. 0 mg•L- 1+ NAA 1. 0 mg•L- 1, 1/ 2 MS+ NAA 1. 0 mg•L- 1+ sugar 1. 5% + carbon3%.
Key words: Aloe vera L., tissue culture, callus, adventitious bud
黄衡宇, 李鹂, 杨胜辉. 芦荟的组织培养[J]. journal6, 2000, 21(3): 11-13.
HUANG Heng-Yu, LI Li, YANG Sheng-Hui. Tissue Culture of Aloe vera L.[J]. journal6, 2000, 21(3): 11-13.
0 / / 推荐
导出引用管理器 EndNote|Ris|BibTeX
链接本文: https://zkxb.jsu.edu.cn/CN/
https://zkxb.jsu.edu.cn/CN/Y2000/V21/I3/11