白及多糖的提取表征及其增效达托霉素的抗菌作用

doi:10.13438/j.cnki.jdzk.2026.02.010

吉首大学学报（自然科学版） ›› 2026, Vol. 47 ›› Issue (2): 72-80.DOI: 10.13438/j.cnki.jdzk.2026.02.010

白及多糖的提取表征及其增效达托霉素的抗菌作用

周兰婷，颜欣平，陈烨，蔡建平，蔡伟凡，王建超，彭华勇

（1.吉首大学药学院，湖南吉首 416000；2.上海加新生物科技有限公司，上海 201210；3.广州珈融化学科技有限公司，广东广州 510530）

出版日期:2026-03-25 发布日期:2026-04-24
作者简介:周兰婷（2002—），女，湖南郴州人，吉首大学药学院硕士研究生，主要从事天然药物抗菌作用机制研究
基金资助:
湖南省自然科学基金资助项目(2025JJ70606)；大学生创新创业训练计划项目(S202510531097，JDCX20251203)

Extraction and Characterization of Bletilla striata Polysaccharide and Its Potentiating Effect on the Antibacterial Activity of Daptomycin

ZHOU Lanting,YAN Xinping,CHEN Ye,CAI Jianping,CAI Weifan,WANG Jianchao,PENG Huayong

(1.School of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China;2.Shanghai Jiaxin Biotechnology Co.,Ltd.,Shanghai 201210,China;3.Guangzhou Jiarong Chemical Technology Co.,Ltd.,Guangzhou 510530,China)

Online:2026-03-25 Published:2026-04-24

摘要/Abstract

摘要：采用热水提取结合醇沉法制备白及多糖（BSP），并通过MCR702，GPC-MALS-RI，SEM等分析BSP分子特征.在此基础上，进一步考察BSP与达托霉素（DAP）的联合抗菌作用.结果表明，BSP得率53.3%，数均分子量58.635 kDa，重均分子量83.319 kDa，多分散性指数1.421，数均旋转半径18.3 nm，重均旋转半径22.5 nm；在盐溶液中，BSP分子呈现紧凑型准球状的构象；在水溶液中，BSP可自发形成多级聚集结构，平均粒径为410.7 nm；BSP（质量浓度1.0 g/L）可使DAP对金黄色葡萄球菌的抗菌活性提高2倍；与DAP单药相比，DAP联用BSP可增强细菌细胞膜通透性，引发核酸、蛋白质、K+等内容物外泄，致使细胞形态皱缩，加速膜电位下降.这提示BSP可通过协同作用促进DAP在细胞膜磷脂中的插入与离子通道的形成，进而发挥杀灭金黄色葡萄球菌的作用.

关键词： 白及多糖, 达托霉素, 抗菌活性, 协同作用, 金黄色葡萄球菌

Abstract: Bletilla striata polysaccharide (BSP) was prepared by hot water extraction combined with alcohol precipitation.The molecular characteristics of BSP were analyzed by MCR702,GPC-MALS-RI,and SEM.On this basis,the combined antibacterial effect of BSP and daptomycin (DAP) was further investigated.The results showed that the yield of BSP was 53.3%,the number average molecular weight was 58.635 kDa,the weight average molecular weight was 83.319 kDa,the polydispersity index was 1.421,the average rotational radius was 18.3 nm,and the weight average radius of gyration was 22.5 nm.In salt solution,the BSP molecules presented a compact and quasi-spherical conformation.In water solution,BSP could spontaneously form multi-level aggregation structures with an average particle size of 410.7 nm.BSP (at a mass concentration of 1.0 g/L) could increase the antibacterial activity of DAP against Staphylococcus aureus by 2 times.Compared with DAP alone,the combination of DAP and BSP could enhance the permeability of bacterial cell membranes,leading to the leakage of nucleic acids,proteins,K+,and other contents,causing cell shrinkage and accelerating the decline of membrane potential.It is suggested that BSP can enhance the killing effect of DAP on Staphylococcus aureus by promoting the insertion of DAP into bacterial cell membrane phospholipids and the formation of ion channels in a synergistic manner.

Key words: Bletilla striata polysaccharide, daptomycin, antibacterial activity, synergy, Staphylococcus aureus

周兰婷, 颜欣平, 陈烨, 蔡建平, 蔡伟凡, 王建超, 彭华勇. 白及多糖的提取表征及其增效达托霉素的抗菌作用[J]. 吉首大学学报（自然科学版）, 2026, 47(2): 72-80.

ZHOU Lanting, YAN Xinping, CHEN Ye, CAI Jianping, CAI Weifan, WANG Jianchao, PENG Huayong. Extraction and Characterization of Bletilla striata Polysaccharide and Its Potentiating Effect on the Antibacterial Activity of Daptomycin[J]. Journal of Jishou University(Natural Sciences Edition), 2026, 47(2): 72-80.

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白及多糖的提取表征及其增效达托霉素的抗菌作用

Extraction and Characterization of Bletilla striata Polysaccharide and Its Potentiating Effect on the Antibacterial Activity of Daptomycin

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